热休克诱导型capR（lon）基因的调控区域：DNA和蛋白质序列。
Regulatory region of the heat shock-inducible capR (lon) gene: DNA and protein sequences.
作者信息
Gayda R C, Stephens P E, Hewick R, Schoemaker J M, Dreyer W J, Markovitz A
出版信息
J Bacteriol. 1985 Apr;162(1):271-5. doi: 10.1128/jb.162.1.271-275.1985.
The CapR protein is an ATP hydrolysis-dependent protease as well as a DNA-stimulated ATPase and a nucleic acid-binding protein. The sequences of the 5' end of the capR (lon) gene DNA and N-terminal end of the CapR protein were determined. The sequence of DNA that specifies the N-terminal portion of the CapR protein was identified by comparing the amino acid sequence of the CapR protein with the sequence predicted from the DNA. The DNA and protein sequences established that the mature protein is not processed from a precursor form. No sequence corresponding to an SOS box was found in the 5' sequence of DNA. There were sequences that corresponded to a putative -35 and -10 region for RNA polymerase binding. The capR (lon) gene was recently identified as one of 17 heat shock genes in Escherichia coli that are positively regulated by the product of the htpR gene. A comparison of the 5' DNA region of the capR gene with that of several other heat shock genes revealed possible consensus sequences.
CapR蛋白是一种依赖ATP水解的蛋白酶，也是一种受DNA刺激的ATP酶和一种核酸结合蛋白。确定了capR（lon）基因DNA的5'端序列和CapR蛋白的N端序列。通过将CapR蛋白的氨基酸序列与从DNA预测的序列进行比较，确定了指定CapR蛋白N端部分的DNA序列。DNA和蛋白质序列表明成熟蛋白不是从前体形式加工而来的。在DNA的5'序列中未发现与SOS框相对应的序列。存在与RNA聚合酶结合的假定-35和-10区域相对应的序列。capR（lon）基因最近被鉴定为大肠杆菌中17个热休克基因之一，这些基因受htpR基因产物的正调控。将capR基因的5'DNA区域与其他几个热休克基因的5'DNA区域进行比较，发现了可能的共有序列。
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