Lon+（capR+）基因产物（一种DNA结合蛋白）的鉴定与纯化。
Identification and purification of the Lon+ (capR+) gene product, a DNA-binding protein.
作者信息
Zehnbauer B A, Foley E C, Henderson G W, Markovitz A
出版信息
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2043-7. doi: 10.1073/pnas.78.4.2043.
Abstract
The polypeptide product of the lon (capR) gene was identified and partially purified from bacterial strains homozygous for the capR(+) or capR9 (ochre mutation) alleles cloned with pSC101. A 94,000-dalton polypeptide was identified as the lon (capR) gene product. Studies of binding to DNA cellulose columns and nitrocellulose filters indicate that the capR(+) and capR9 proteins bind DNA.
摘要
从用pSC101克隆的capR(+)或capR9（赭石突变）等位基因纯合的细菌菌株中鉴定并部分纯化了lon（capR）基因的多肽产物。一种94,000道尔顿的多肽被鉴定为lon（capR）基因产物。与DNA纤维素柱和硝酸纤维素滤膜结合的研究表明，capR(+)和capR9蛋白能结合DNA。
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