Burkovski A, Weil B, Krämer R
Forschungszentrum Jülich GmbH, Institut für Biotechnologie I, Germany.
Arch Microbiol. 1995 Jul;164(1):24-8. doi: 10.1007/BF02568730.
Glutamate excretion due to amino acid starvation was investigated in "stringent" and "relaxed" strains of Escherichia coli. The observed excretion process is relA-dependent, carrier-mediated, and glutamate-specific. After induction, excretion was detected within less than 2 min and continued for more than 5 h with a rate of 7-10 nmol (mg dry weight)-1 min-1. Using carbonyl cyanide m-chlorophenylhydrazone or polymyxin B nonapeptide, together with valinomycin, it was shown that glutamate excretion is driven by the membrane potential.
在大肠杆菌的“严谨型”和“松弛型”菌株中研究了氨基酸饥饿导致的谷氨酸排泄。观察到的排泄过程依赖于relA，由载体介导，且具有谷氨酸特异性。诱导后，在不到2分钟内即可检测到排泄，并以7 - 10 nmol（毫克干重）-1分钟-1的速率持续超过5小时。使用羰基氰化物间氯苯腙或多粘菌素B九肽与缬氨霉素一起，结果表明谷氨酸排泄是由膜电位驱动的。
