Wolny-Koładka Katarzyna A, Malina Dagmara K
a Department of Microbiology , University of Agriculture in Cracow , Poland.
b Faculty of Chemical Engineering and Technology, Cracow University of Technology , Poland.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2018;53(12):1041-1047. doi: 10.1080/10934529.2018.1474568. Epub 2018 May 29.
The aim of this study was to assess bactericidal properties of nanosilver obtained with ascorbic acid (vitamin C) as a reducing substance, against environmental strains of Gram-positive (Staphylococcus) and Gram-negative bacteria (Escherichia coli). Silver nanoparticles were obtained by a simple and fast method of chemical reduction in mild synthesis condition using substrates not classified as dangerous and commonly found in plants. Bacterial susceptibility to nanosilver was determined using the disk-diffusion method. Gram-positive bacteria were less susceptible to bactericidal action of nanosilver and minimum inhibitory concentration was higher for these bacteria. However, susceptibility of individual isolates of different species to nanosilver was very diverse. The range of growth inhibition zones indicates that the resistance to different concentrations of nanosilver was a strain-characteristic, not species-related feature. The study of effective nanomaterials synthesis and applicability appears to be an extremely important element in the development of nanotechnology, especially in terms of the risks assessment. Obtained results have a real chance to be an important step toward creating a new generation of disinfectants without toxic effects on the environment and higher organisms.
本研究的目的是评估以抗坏血酸（维生素C）作为还原物质制得的纳米银对革兰氏阳性菌（葡萄球菌）和革兰氏阴性菌（大肠杆菌）环境菌株的杀菌性能。纳米银颗粒是在温和的合成条件下，采用一种简单快速的化学还原方法制得的，所使用的底物并非危险物质，且在植物中普遍存在。采用纸片扩散法测定细菌对纳米银的敏感性。革兰氏阳性菌对纳米银的杀菌作用较不敏感，这些细菌的最低抑菌浓度较高。然而，不同物种的各个分离株对纳米银的敏感性差异很大。生长抑制圈的范围表明，对不同浓度纳米银的抗性是菌株特有的特征，而非物种相关的特征。有效的纳米材料合成及适用性研究似乎是纳米技术发展中极其重要的一个因素，尤其是在风险评估方面。所获得的结果很有可能成为朝着开发对环境和高等生物无毒性影响的新一代消毒剂迈出的重要一步。
