关于从全基因组测序数据中确定利奈唑胺耐药性所遇到的困难的建议。
Recommendations To Address the Difficulties Encountered When Determining Linezolid Resistance from Whole-Genome Sequencing Data.
机构信息
Department of Microbiology and Infectious Diseases, Royal Prince Alfred Hospital, Sydney, NSW, Australia
Statens Serum Institut, Department of Bacteria, Parasites, and Fungi, Copenhagen, Denmark.
出版信息
Antimicrob Agents Chemother. 2018 Jul 27;62(8). doi: 10.1128/AAC.00613-18. Print 2018 Aug.
Abstract
Mutations associated with linezolid resistance within the V domain of 23S rRNA are annotated using an numbering system. The 23S rRNA gene varies in length, nucleotide sequence, and copy number among bacterial species. Consequently, this numbering system is not intuitive and can lead to confusion when mutation sites are being located using whole-genome sequencing data. Using the mutation G2576T as an example, we demonstrate the difficulties associated with using the numbering system.
摘要
与 23S rRNA V 结构域中的利奈唑胺耐药相关的突变使用数字编号系统进行注释。23S rRNA 基因在长度、核苷酸序列和拷贝数方面在不同细菌物种中存在差异。因此，这种编号系统不直观，并且在使用全基因组测序数据定位突变位点时可能会导致混淆。我们以突变 G2576T 为例，展示了使用数字编号系统所面临的困难。
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