Gargis Amy S, Spicer Lori M, Kent Alyssa G, Zhu Wenming, Campbell Davina, McAllister Gillian, Ewing Thomas O, Albrecht Valerie, Stevens Valerie A, Sheth Mili, Padilla Jasmine, Batra Dhwani, Johnson J Kristie, Halpin Alison Laufer, Rasheed J Kamile, Elkins Christopher A, Karlsson Maria, Lutgring Joseph D
Division of Healthcare Quality Promotion, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, United States.
Goldbelt C6, LLC, Chesapeake, VA, United States.
Front Microbiol. 2022 Feb 1;12:807398. doi: 10.3389/fmicb.2021.807398. eCollection 2021.
and with resistance to daptomycin and/or linezolid are emerging globally. We present the genomic characterization of daptomycin- and linezolid-resistant and surveillance isolates from the United States, 2013-2016. Daptomycin resistance was low among (2/364, 0.5%) and (17/344, 5%). The majority (71%, 12/17) of daptomycin-resistant isolates belonged to the emerging ST736 clone and contained mutations in and previously associated with resistance. However, 1/2 and 3/17 did not contain these mutations previously associated with daptomycin resistance. Linezolid resistance was rare among (1/364, 0.3%) and (2/344, 0.6%). These two isolates, one of which was also resistant to daptomycin and vancomycin, contained the 23S rRNA nucleotide mutation (G2576T) associated with linezolid resistance. Long-read sequencing revealed the linezolid-resistant isolate contained chromosomal- and plasmid-encoded copies of . The chromosomal was located on the recently described Tn multiresistance transposon. The second copy of was encoded on an ∼65 kb mosaic plasmid, with component regions sharing high sequence identity to -encoding multiresistance plasmids of animal origin. The -encoding plasmid contained open reading frames predicted to encode proteins associated with a pheromone-responsive plasmid transfer system, and filter mating experiments confirmed the plasmid was conjugative. Continued surveillance of enterococci is necessary to assess the prevalence and trends of daptomycin and linezolid resistance in the United States, characterize resistance mechanisms and how they transfer, and monitor for emerging sequence types associated with resistance.
并且对达托霉素和/或利奈唑胺耐药的情况正在全球范围内出现。我们展示了2013 - 2016年来自美国的耐达托霉素和耐利奈唑胺监测分离株的基因组特征。达托霉素耐药在屎肠球菌中较低（2/364，0.5%），在粪肠球菌中为17/344（5%）。大多数（71%，12/17）耐达托霉素的屎肠球菌分离株属于新兴的ST736克隆，并且在先前与耐药相关的基因和基因中含有突变。然而，2株屎肠球菌中的1株以及17株粪肠球菌中的3株并不含有这些先前与达托霉素耐药相关的突变。利奈唑胺耐药在屎肠球菌中罕见（1/364，0.3%），在粪肠球菌中为2/344（0.6%）。这两株粪肠球菌分离株，其中一株也对达托霉素和万古霉素耐药，含有与利奈唑胺耐药相关的23S rRNA核苷酸突变（G2576T）。长读长测序显示耐利奈唑胺的粪肠球菌分离株含有染色体编码和质粒编码的基因拷贝。染色体基因位于最近描述的Tn多耐药转座子上。基因的第二个拷贝编码在一个约65 kb的嵌合质粒上，其组成区域与动物源编码多耐药质粒具有高度序列同一性。编码基因的质粒包含预测编码与信息素响应质粒转移系统相关蛋白质的开放阅读框，滤膜交配实验证实该质粒是可接合的。持续监测肠球菌对于评估美国达托霉素和利奈唑胺耐药的流行情况和趋势、表征耐药机制及其转移方式以及监测与耐药相关的新兴序列类型是必要的。
