Obermannova Radka, Redova-Lojova Martina, Vychytilova-Faltejskova Petra, Grell Peter, Cho William C, Sachlova Milana, Svoboda Marek, Vyzula Rostislav, Slaby Ondrej
Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Brno, Czech Republic.
Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
Anticancer Res. 2018 Jun;38(6):3719-3724. doi: 10.21873/anticanres.12651.
BACKGROUND/AIM: In Western countries, most patients with gastric cancer (GC) present in advanced stages. Therefore, there is imminent clinical need for novel diagnostic and prognostic biomarkers. Deregulation of microRNAs has been reported as a frequent event in GC development in a number of studies. Our study validated the potential of microRNAs to serve as diagnostic and prognostic biomarkers in patients with GC from the Central European population.
Using quantitative real-time polymerase chain reaction, expression levels of six microRNAs (miR-10b, -21, -93, -107, - 143, and -145) were examined in 67 tumor tissues and 67 paired adjacent gastric tissues, and correlated with clinicopathological features of GC patients.
Expression levels of miR-10b, miR-21, miR-93, and miR-107 were significantly higher in GC samples compared to non-tumor tissue. Furthermore, the expression levels of miR-10b, miR-143, and miR-145 positively correlated with advanced stages, and increased expression of miR-10b, miR-21 and miR-145 was significantly associated with worse prognosis of gastric cancer patients.
Our results indicate that selected tissue microRNAs have the potential to serve as relevant diagnostic and prognostic biomarkers of GC in a central European population.
背景/目的：在西方国家，大多数胃癌（GC）患者就诊时已处于晚期。因此，临床上迫切需要新的诊断和预后生物标志物。多项研究报道，微小RNA的失调在胃癌发生过程中是常见事件。我们的研究验证了微小RNA作为中欧人群胃癌患者诊断和预后生物标志物的潜力。
采用定量实时聚合酶链反应，检测67例肿瘤组织和67例配对的相邻胃组织中6种微小RNA（miR-10b、-21、-93、-107、-143和-145）的表达水平，并与胃癌患者的临床病理特征进行相关性分析。
与非肿瘤组织相比，胃癌样本中miR-10b、miR-21、miR-93和miR-107的表达水平显著升高。此外，miR-10b、miR-143和miR-145的表达水平与晚期呈正相关，miR-10b、miR-21和miR-145表达增加与胃癌患者预后较差显著相关。
我们的结果表明，所选组织微小RNA有潜力作为中欧人群胃癌相关的诊断和预后生物标志物。
