State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Biomed Res Int. 2018 Apr 18;2018:1523147. doi: 10.1155/2018/1523147. eCollection 2018.
The high within-litter birth weight variation has become a big issue in multiparous animals. The present study was conducted to investigate the effects of leucine supplementation in premating diet on the reproductive performance, maternal antioxidative capability, and immune function in primiparous rats. Six-week-old female SD rats were assigned to basal diet or 0.6% leucine supplemented diet for two weeks. After mating during the eighth week of age, the rats were fed with regular gestation diet. Maternal blood samples were collected on the day before mating (day -1) and day 7 and day 20 of pregnancy, while ovaries and uteruses were obtained on day -1 and on day 7, respectively. The results indicate that, compared with control group, within-litter birth weight variation was significantly decreased, while birth weights were significantly increased in the leucine group ( < 0.01). Also, leucine improved the embryo distribution uniformity and the number of implantation sites in uterine. The ovarian gene expressions of , , and were upregulated, while was decreased significantly ( < 0.05). Leucine also increased the maternal antioxidant capacity and immune function. Conclusively, leucine supplementation in premating diet could improve the reproductive performance, which could be attributed to the improved oxidative and immune status.
胎内初生重变异性高已成为多胎动物的一个大问题。本研究旨在探讨在配种前饲料中添加亮氨酸对初产大鼠繁殖性能、母体抗氧化能力和免疫功能的影响。6 周龄雌性 SD 大鼠被分配到基础饮食或 0.6%亮氨酸补充饮食中，持续两周。在 8 周龄时交配后，大鼠被喂食常规妊娠饮食。在配种前 1 天（-1 天）和妊娠第 7 天和第 20 天采集母鼠血液样本，同时在-1 天和第 7 天采集卵巢和子宫。结果表明，与对照组相比，亮氨酸组胎内初生重变异性显著降低，初生重显著增加（<0.01）。此外，亮氨酸还改善了胚胎在子宫内的分布均匀性和着床点数量。卵巢基因的表达上调，而 表达显著下调（<0.05）。亮氨酸还提高了母体的抗氧化能力和免疫功能。综上所述，配种前饲料中添加亮氨酸可改善繁殖性能，这可能归因于氧化和免疫状态的改善。
