Huang Ruojing, Liang Shaomin, Fang Lyujie, Wu Min, Cheng Huanhuan, Mi Xuesong, Ding Yong
Department of Ophthalmology, The First Affiliated Hospital of Jinan University, Guangzhou, China.
Department of Ophthalmology, Guangzhou first people's hospital, Guangzhou, China.
Mol Vis. 2018 May 18;24:367-378. eCollection 2018.
The aim of this study was to investigate the effect of minocycline (MC) on the survival of retinal ganglion cells (RGCs) in an ischemic-reperfusion (I/R) injury model of retinal degeneration.
Retinal I/R injury was induced in the left eye of mice for 60 min by maintaining intraocular pressure at 90 mmHg. Low- or high-dose MC (20 or 100 mg/kg, respectively) was administered by intravenous injection at 5 min after the retinal ischemic insult and then administered once daily until the mice were euthanized. RGCs and microglial cells were counted using immunofluorescence staining. Functional changes in the RGCs were evaluated using electroretinography. The visual function was assessed using an optokinetic test.
The data demonstrated that the effect of MC was dose dependent. Low-dose MC showed protective effects, with reduced RGC loss and microglial activation, while the high-dose MC showed damage effects, with more RGC loss and microglial activation when compared with the vehicle group. The electroretinography and optokinetic test results were consistent with the morphologic observations.
These data suggested that appropriate concentrations of MC can protect the retina against retinal ischemic-reperfusion injury, while excessive MC has detrimental effects.
本研究旨在探讨米诺环素（MC）对视网膜变性缺血再灌注（I/R）损伤模型中视网膜神经节细胞（RGCs）存活的影响。
通过将小鼠左眼眼压维持在90 mmHg诱导视网膜I/R损伤60分钟。在视网膜缺血损伤后5分钟通过静脉注射给予低剂量或高剂量MC（分别为20或100 mg/kg），然后每天给药一次，直至小鼠安乐死。使用免疫荧光染色对RGCs和小胶质细胞进行计数。使用视网膜电图评估RGCs的功能变化。使用视动性试验评估视觉功能。
数据表明MC的作用具有剂量依赖性。低剂量MC显示出保护作用，RGC损失减少，小胶质细胞活化降低，而高剂量MC显示出损伤作用，与载体组相比，RGC损失更多，小胶质细胞活化增加。视网膜电图和视动性试验结果与形态学观察结果一致。
这些数据表明，适当浓度的MC可保护视网膜免受视网膜缺血再灌注损伤，而过量的MC则具有有害作用。
