Ridwan Rasdin, Abdul Razak Hairil Rashmizal, Adenan Mohd Ilham, Md Saad Wan Mazlina
Centre of Medical Laboratory Technology, Faculty of Health Sciences, Universiti Teknologi MARA, Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor, Malaysia.
Centre of Medical Imaging, Faculty of Health Sciences, Universiti Teknologi MARA, Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor, Malaysia.
Int J Anal Chem. 2018 May 2;2018:4798530. doi: 10.1155/2018/4798530. eCollection 2018.
Watermelons are known to have sufficient amino acid content. In this study, watermelons grown and consumed in Malaysia were investigated for their amino acid content, L-citrulline and L-arginine, by the isocratic RP-HPLC method. Flesh and rind watermelons were juiced, and freeze-dried samples were used for separation and quantification of L-citrulline and L-arginine. Three different mobile phases, 0.7% HP0, 0.1% HP0, and 0.7% HP0 : ACN (90 : 10), were tested on two different columns using Zorbax Eclipse XDB-C and Gemini C with a flow rate of 0.5 mL/min and a detection wavelength at 195 nm. Efficient separation with reproducible resolution of L-citrulline and L-arginine was achieved using 0.1% HP0 on the Gemini C column. The method was validated and good linearity of L-citrulline and L-arginine was obtained with = 0.9956, = 0.1664 + 2.4142 and = 0.9912, = 0.4100 + 3.4850, respectively. L-citrulline content showed the highest concentration in red watermelon of flesh and rind juice extract (43.81 mg/g and 45.02 mg/g), whereas L-arginine concentration was lower than L-citrulline, ranging from 3.39 to 11.14 mg/g. The isocratic RP-HPLC method with 0.1% HP0 on the Gemini C column proved to be efficient for separation and quantification of L-citrulline and L-arginine in watermelons.
众所周知，西瓜含有丰富的氨基酸。在本研究中，采用等度反相高效液相色谱法（RP-HPLC）对马来西亚种植和食用的西瓜的氨基酸含量、L-瓜氨酸和L-精氨酸进行了研究。将西瓜果肉和瓜皮榨汁，冻干后的样品用于L-瓜氨酸和L-精氨酸的分离和定量分析。使用Zorbax Eclipse XDB-C和Gemini C两种不同的色谱柱，测试了三种不同的流动相，即0.7%的磷酸（HP0）、0.1%的磷酸（HP0）以及0.7%的磷酸（HP0）：乙腈（ACN）（90：10），流速为0.5 mL/min，检测波长为195 nm。在Gemini C色谱柱上使用0.1%的磷酸（HP0）实现了L-瓜氨酸和L-精氨酸的高效分离，且分离度具有可重复性。该方法经过验证，L-瓜氨酸和L-精氨酸具有良好的线性关系，相关系数分别为r = 0.9956，y = 0.1664 + 2.4142x以及r = 0.9912，y = 0.4100 + 3.4850x。L-瓜氨酸含量在红肉西瓜的果肉和瓜皮汁提取物中浓度最高（分别为43.81 mg/g和45.02 mg/g），而L-精氨酸浓度低于L-瓜氨酸，范围在3.39至11.14 mg/g之间。结果表明，在Gemini C色谱柱上使用0.1%的磷酸（HP0）的等度反相高效液相色谱法对于西瓜中L-瓜氨酸和L-精氨酸的分离和定量分析是有效的。
