Poormoosavi Seyedeh Mahsa, Najafzadehvarzi Hosein, Behmanesh Mohammad Amin, Amirgholami Reza
Department of Histology, School of Medicine, Dezful University of Medical Science, Dezful, Iran.
Department of Pharmacology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
Toxicol Rep. 2018 Mar 9;5:427-433. doi: 10.1016/j.toxrep.2018.02.010. eCollection 2018.
is an herbal medicine with remarkable antioxidant, anti-inflammatory, and antihepatotoxic properties. The present study aimed to investigate whether Bisphenol A (BPA) could induce histopathological changes and oxidative stress in the liver and kidney tissues of male rats. In addition, we evaluated whether the co-administration of the extract (AOE) could prevent the possible damages. In total, 40 adult male Wistar rats with the mean weight of 200 ± 20 grams were divided into five groups. Control subjects were placed in the first group, while group two was the vehicle (5 ml/kg bwt/day). Subjects in group three were administered with 400 mg/kg of AOE (bwt/day), group four received 10 mg/kg of BPA (bwt/day) dissolved in 5 ml/kg of olive oil, and group five received oral BPA and AOE daily for eight weeks. After the experiments, the blood, liver, and kidneys of the animals were collected and examined. Biochemical results showed a significant elevation in the levels of liver and kidney biomarkers in the BPA group (P ≤ 0.05). Moreover, malondialdehyde was observed to increase, while thiol protein and total antioxidant capacity decreased. Histopathological results of the BPA group indicated dilated and congested central and portal veins and inflammatory areas in the liver. In addition, renal test results showed casts in the urinary tubules and acute tubular necrosis. According to the results, the co-administration of AOE and BPA could increase the total antioxidative capability, thereby improving the function and structure of the liver and kidney tissues. Therefore, AOE is a potential protective agent against oxidative stress, liver, and kidney damage.
是一种具有显著抗氧化、抗炎和抗肝毒性特性的草药。本研究旨在调查双酚A（BPA）是否会在雄性大鼠的肝脏和肾脏组织中诱导组织病理学变化和氧化应激。此外，我们评估了提取物（AOE）的联合给药是否可以预防可能的损伤。总共40只平均体重为200±20克的成年雄性Wistar大鼠被分为五组。对照组置于第一组，而第二组为赋形剂组（5毫升/千克体重/天）。第三组的大鼠给予400毫克/千克的AOE（体重/天），第四组接受溶解于5毫升/千克橄榄油中的10毫克/千克BPA（体重/天），第五组大鼠连续八周每天口服BPA和AOE。实验结束后，收集并检查动物的血液、肝脏和肾脏。生化结果显示，BPA组肝脏和肾脏生物标志物水平显著升高（P≤0.05）。此外，观察到丙二醛增加，而硫醇蛋白和总抗氧化能力下降。BPA组的组织病理学结果表明肝脏中央静脉和门静脉扩张、充血以及存在炎症区域。此外，肾脏检测结果显示肾小管中有管型和急性肾小管坏死。根据结果，AOE与BPA联合给药可提高总抗氧化能力，从而改善肝脏和肾脏组织的功能和结构。因此，AOE是一种针对氧化应激、肝脏和肾脏损伤的潜在保护剂。
