Inspirotec Inc, Chicago, Illinois.
Kenilworth Medical Allergy & Immunology, Kenilworth, Illinois.
Ann Allergy Asthma Immunol. 2018 Aug;121(2):211-217.e3. doi: 10.1016/j.anai.2018.05.024. Epub 2018 Jun 1.
In current practice, allergens in vacuum-collected dust are used as surrogates for inhalable allergens. We developed an air-sampling device that can be used by patients for direct measurement of airborne allergen concentrations in their own homes.
To demonstrate the use of this device to establish allergen concentration reference ranges in a target population and to evaluate associations between patient-reported information and measured allergen concentrations.
Patients from 5 allergist's practices in the Chicagoland region were provided with instructions, questionnaires, informed consent forms, and samplers to run for 5 days in their bedrooms. Samples were collected from cartridges and assayed by multiplex immunoassays for 12 common household allergens and enzyme-linked immunosorbent assay for ragweed.
Unique allergen profiles were obtained for 102 patient homes. Samples with allergen concentrations above the limit of detection were as follows: total dust mite, 28%; cat, 61%; dog, 64%; mouse, 12%; rat, 0%; cockroach, 4%; Alternaria, 6%; Aspergillus, 21%; birch pollen 1%; grass, 8%; and ragweed, 5%. Of those, 75 completed questionnaires, providing meta-data for further analysis. Pet allergens correlated significantly with number of pets owned. Humidity correlated with dust mite allergens, open windows with Alternaria and mouse allergens, and high-efficiency particulate air filter use with reduced levels of several allergens. Many other variables showed no significant correlations.
The combination of ease of use, high air-sampling rate, and sensitive immunoassays permitted the measurement of airborne allergen concentrations in homes and establishment of reference ranges. Patient-reported information permitted identification of factors that could relate to allergen concentrations and suggested remedial measures.
在当前的实践中，真空采集的灰尘过敏原被用作可吸入过敏原的替代品。我们开发了一种空气采样装置，可供患者在家中直接测量空气中过敏原的浓度。
展示该设备在目标人群中建立过敏原浓度参考范围的使用，并评估患者报告的信息与测量的过敏原浓度之间的关联。
芝加哥地区 5 家过敏科诊所的患者收到了说明书、问卷、知情同意书和采样器，以便在卧室中运行 5 天。从试剂盒中采集样本，并用多重免疫分析法测定 12 种常见的室内过敏原，酶联免疫吸附法测定豚草。
为 102 个患者家庭获得了独特的过敏原谱。过敏原浓度超过检测限的样本如下：总尘螨，28%；猫，61%；狗，64%；鼠，12%；大鼠，0%；蟑螂，4%；交链孢霉，6%；曲霉，21%；桦树花粉，1%；草，8%；豚草，5%。其中，75 人完成了问卷调查，提供了进一步分析的元数据。宠物过敏原与宠物数量显著相关。湿度与尘螨过敏原相关，开窗户与交链孢霉和鼠过敏原相关，高效空气过滤器的使用与几种过敏原水平降低相关。许多其他变量没有显示出显著相关性。
使用方便、高空气采样率和灵敏的免疫分析相结合，允许在家中测量空气中的过敏原浓度并建立参考范围。患者报告的信息允许识别与过敏原浓度相关的因素，并提出补救措施。
