Yusan Sabriye, Rahman Mokhlesur M, Mohamad Nasir, Arrif Tengku M, Latif Ahmad Zubaidi A, M A Mohd Aznan, Wan Nik Wan Sani B
Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia.
Institute of Nuclear Science, Ege University, Bornova, 35100 Izmir, Turkey.
J Anal Methods Chem. 2018 May 10;2018:2687341. doi: 10.1155/2018/2687341. eCollection 2018.
A new bioenzymatic glucose biosensor for selective and sensitive detection of glucose was developed by the immobilization of glucose oxidase (GOD) onto selenium nanoparticle-mesoporous silica composite (MCM-41) matrix and then prepared as a carbon paste electrode (CPE). Cyclic voltammetry was employed to probe the catalytic behavior of the biosensor. A linear calibration plot is obtained over a wide concentration range of glucose from 1 × 10 to 2 × 10 M. Under optimal conditions, the biosensor exhibits high sensitivity (0.34 A·mM), low detection limit (1 × 10 M), high affinity to glucose (K = 0.02 mM), and also good reproducibility (R.S.D. 2.8%, =10) and a stability of about ten days when stored dry at +4°C. Besides, the effects of pH value, scan rate, mediator effects on the glucose current, and electroactive interference of the biosensor were also discussed. As a result, the biosensor exhibited an excellent electrocatalytic response to glucose as well as unique stability and reproducibility.
通过将葡萄糖氧化酶（GOD）固定在硒纳米颗粒 - 介孔二氧化硅复合材料（MCM - 41）基质上，然后制备成碳糊电极（CPE），开发了一种用于选择性和灵敏检测葡萄糖的新型生物酶葡萄糖生物传感器。采用循环伏安法探究该生物传感器的催化行为。在1×10至2×10 M的宽葡萄糖浓度范围内获得了线性校准曲线。在最佳条件下，该生物传感器具有高灵敏度（0.34 A·mM）、低检测限（1×10 M）、对葡萄糖的高亲和力（K = 0.02 mM），并且还具有良好的重现性（相对标准偏差2.8%，n = 10），在 +4°C干燥保存时稳定性约为十天。此外，还讨论了pH值、扫描速率、介质对葡萄糖电流的影响以及生物传感器的电活性干扰。结果表明，该生物传感器对葡萄糖表现出优异的电催化响应以及独特的稳定性和重现性。
