Department of Dentistry, National Yang-Ming University, No. 155, Sec. 2, Linong St., Beitou District, Taipei 112, Taiwan.
Department of Stomatology, Veterans General Hospital Taipei, No. 201, Section 2, Shipai Road, Beitou District, Taipei City 112, Taiwan.
Biomed Res Int. 2018 May 15;2018:3240571. doi: 10.1155/2018/3240571. eCollection 2018.
The aim of this research is to synthesize polycaprolactone-based polyurethanes (PCL-based PUs) that can be further used for the fabrication of guided bone regeneration (GBR) membranes with higher tensile strength and elongation at break than collagen and PTFE membranes. The PCL-based PUs were prepared by the polymerization of polycaprolactone (PCL) diol with 1,6-hexamethylene diisocyanate (HDI) at different ratios using either polyethylene glycol (PEG) or ethylenediamine (EDA) as chain extenders. The chemical, mechanical, and thermal properties of the synthesized polymers were determined using NMR, FTIR, GPC, DSC, and tensile tester. The PCL and polyurethanes were fabricated as nanofiber membranes by electrospinning, and their mechanical properties and SEM morphology were also investigated. tests, including WST-1 assay, SEM of cells, and phalloidin cytoskeleton staining, were also performed. It was shown that electrospun membranes made of PCL and PCL-HDI-PEG (2 : 3 : 1) possessed tensile strength of 19.84 MPa and 11.72 MPa and elongation at break of 627% and 362%, respectively. These numbers are equivalent or higher than most of the commercially available collagen and PTFE membrane. As a result, these membranes may have potential for future GBR applications.
本研究旨在合成聚己内酯基聚氨酯（PCL 基 PU），可进一步用于制备拉伸强度和断裂伸长率高于胶原和 PTFE 膜的引导骨再生（GBR）膜。通过聚己内酯二醇（PCL）与 1,6-己二异氰酸酯（HDI）在不同比例下聚合，使用聚乙二醇（PEG）或乙二胺（EDA）作为扩链剂，制备了 PCL 基 PU。采用 NMR、FTIR、GPC、DSC 和拉伸试验机测定了合成聚合物的化学、力学和热性能。通过静电纺丝将 PCL 和聚氨酯制成纳米纤维膜，并对其力学性能和 SEM 形貌进行了研究。还进行了包括 WST-1 测定、细胞 SEM 和鬼笔环肽细胞骨架染色在内的 测试。结果表明，由 PCL 和 PCL-HDI-PEG（2：3：1）制成的静电纺丝膜的拉伸强度分别为 19.84 MPa 和 11.72 MPa，断裂伸长率分别为 627%和 362%。这些数值与大多数市售胶原和 PTFE 膜相当或更高。因此，这些膜可能具有用于未来 GBR 应用的潜力。
