Zhu Hongni, St Dollente Mesias Vince, Dai Xin, Qiu Wenting, Yao Xiaobin, Huang Jinqing
Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China.
Research Center for Biomedical Optics and Molecular Imaging, Key Laboratory of Biomedical Imaging Science and System, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.
Anal Bioanal Chem. 2025 May 22. doi: 10.1007/s00216-025-05903-2.
Leveraging fingerprint-like specificity of molecular vibrations and enhanced sensitivity from metallic nanostructures, surface-enhanced Raman spectroscopy (SERS) has emerged as a powerful analytical tool. However, most SERS substrates are designed for disposable single-use, which compromises data reproducibility, increases operational costs, raises sustainability concerns, and narrows practical applications. Furthermore, the complex fabrication methods of existing reusable SERS substrates and their limited recycling/reusable cycles pose significant challenges to address the needs of automated high-throughput analytical applications. In this study, we developed a novel nanoporous silver sheet as a background-free, highly sensitive, and reusable SERS platform, using a simple chemical redox method. With multiple layers of uniform coral rock-like nano-silver rods, it demonstrated excellent sensitivity with a detection limit of 1 × 10⁻⁷ M (24 ppb) for thiram and universal detection capability across a diverse range of 10 pesticides, such as carbamates, organophosphorus compounds, pyrethroids, organochlorines, and benzimidazoles, which was further validated through practical testing on yellow cabbages. More importantly, this nanoporous silver sheet could be reused at least 60 times from a straightforward rinsing procedure to maintain its consistently outstanding performance for continues SERS measurements. Given its accessibility and economic viability, this highly reusable SERS substrate holds the promise to promote the practical applications of SERS in various fields in the future.
利用分子振动的指纹状特异性以及金属纳米结构增强的灵敏度，表面增强拉曼光谱（SERS）已成为一种强大的分析工具。然而，大多数SERS基底设计为一次性使用，这损害了数据的可重复性，增加了运营成本，引发了可持续性问题，并限制了实际应用。此外，现有可重复使用的SERS基底复杂的制造方法及其有限的回收/可重复使用循环，对满足自动化高通量分析应用的需求构成了重大挑战。在本研究中，我们使用简单的化学氧化还原方法，开发了一种新型纳米多孔银片作为无背景、高灵敏度且可重复使用的SERS平台。它具有多层均匀的珊瑚岩状纳米银棒，对福美双表现出优异的灵敏度，检测限为1×10⁻⁷ M（24 ppb），并且对多种10种农药具有通用检测能力，如氨基甲酸酯类、有机磷化合物、拟除虫菊酯类、有机氯类和苯并咪唑类，通过在黄心菜上的实际测试进一步验证。更重要的是，这种纳米多孔银片通过简单的冲洗程序至少可重复使用60次，以保持其在连续SERS测量中始终出色的性能。鉴于其可及性和经济可行性，这种高度可重复使用的SERS基底有望在未来促进SERS在各个领域的实际应用。
