白细胞中 ADRB3 基因的 DNA 甲基化水平降低与营养良好的成年人血清叶酸有关。
Decrease of the DNA methylation levels of the ADRB3 gene in leukocytes is related with serum folate in eutrophic adults.
机构信息
Health Sciences Center (Centro de Ciências da Saúde), Departament of Nutrition, Federal University of Paraíba (Universidade Federal da Paraíba), João Pessoa, Brazil.
Center of Exact Sciences and Nature (Centro de Ciências Exatas e da Natureza), Federal University of Paraíba (Universidade Federal da Paraíba), João Pessoa, Brazil.
出版信息
J Transl Med. 2018 Jun 5;16(1):152. doi: 10.1186/s12967-018-1529-0.
BACKGROUND
DNA methylation has been evidenced as a potential epigenetic mechanism related to various candidate genes to development of obesity. Therefore, the objective of this study was to evaluate the DNA methylation levels of the ADRB3 gene by body mass index (BMI) in a representative adult population, besides characterizing this population as to the lipid profile, oxidative stress and food intake.
METHODS
This was a cross-sectional population-based study, involving 262 adults aged 20-59 years, of both genders, representative of the East and West regions of the municipality of João Pessoa, Paraíba state, Brazil, in that were evaluated lifestyle variables and performed nutritional, biochemical evaluation and DNA methylation levels of the ADRB3 gene using high resolution melting method. The relationship between the study variables was performed using analyses of variance and multiple regression models. All results were obtained using the software R, 3.3.2.
RESULTS
From the stratification of categories BMI, was observed a difference in the average variables values of age, waist-to-height ratio, waist-to-hip ratio, waist circumference, triglycerides and intake of trans fat, which occurred more frequently between the categories "eutrophic" and "obesity". From the multiple regression analysis in the group of eutrophic adults, it was observed a negative relationship between methylation levels of the ADRB3 gene with serum levels of folic acid. However, no significant relation was observed among lipid profile, oxidative stress and food intake in individuals distributed in the three categories of BMI.
CONCLUSIONS
A negative relationship was demonstrated between methylation levels of the ADRB3 gene in eutrophic adults individuals with serum levels of folic acid, as well as with the independent gender of BMI, however, was not observed relation with lipid profile, oxidative stress and variables of food intake. Regarding the absence of relationship with methylation levels of the ADRB3 gene in the categories of overweight, mild and moderate obesity, the answer probably lies in the insufficient amount of body fat to initiate inflammatory processes and oxidative stress with a direct impact on methylation levels, what is differently is found most of the times in exacerbated levels in severe obesity.
背景
DNA 甲基化已被证明是与肥胖相关的各种候选基因的潜在表观遗传机制。因此，本研究的目的是评估 ADRB3 基因的 DNA 甲基化水平与体重指数（BMI）的关系，同时对该人群的脂质谱、氧化应激和饮食摄入进行特征描述。
方法
这是一项基于人群的横断面研究，涉及巴西帕拉伊巴州若昂佩索阿市东西区的 262 名 20-59 岁的成年男女，评估了生活方式变量，并采用高分辨率熔解法对 ADRB3 基因的 DNA 甲基化水平进行了营养、生化评估。使用方差分析和多元回归模型对研究变量之间的关系进行了分析。所有结果均使用 R 软件 3.3.2 获得。
结果
从 BMI 分类的分层来看，在年龄、腰高比、腰臀比、腰围、甘油三酯和反式脂肪摄入量等平均变量值方面存在差异，这些差异在“正常体重”和“肥胖”类别之间更为常见。在正常体重成年人的多元回归分析中，观察到 ADRB3 基因的甲基化水平与血清叶酸水平呈负相关。然而，在 BMI 分布在三个类别的个体中，未观察到脂质谱、氧化应激和饮食摄入之间存在显著关系。
结论
在正常体重成年人中，ADRB3 基因的甲基化水平与血清叶酸水平呈负相关，与 BMI 的独立性别呈负相关，但与脂质谱、氧化应激和饮食摄入变量无关。至于超重、轻度和中度肥胖类别的 ADRB3 基因甲基化水平与体重指数之间不存在关系，答案可能在于体内脂肪量不足以引发炎症和氧化应激过程，从而对甲基化水平产生直接影响，而在严重肥胖中，这种情况更常见。
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