Wang Jian, Leiva Sergio, Huang Jiao, Huang Ying
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Instituto de Bioquímica & Microbiología, Facultad de Ciencias, Universidad Austral de Chile, Casilla 567, Valdivia, Chile.
Int J Syst Evol Microbiol. 2018 Jul;68(7):2348-2356. doi: 10.1099/ijsem.0.002844. Epub 2018 Jun 5.
Two moderately psychrophilic actinobacterial strains, designated AU-G6 and AU-A3.2, isolated from the surface of an Antarctic macroalga, Adenocystis utricularis (Bory) Skottsberg, was taxonomically characterized based on a polyphasic investigation. The two strains had nearly identical 16S rRNA gene sequences and formed a distinct phyletic line within the genus Amycolatopsis of the family Pseudonocardiaceae. They were phylogenetically close to Amycolatopsis nigrescens JCM 14717, Amycolatopsis minnesotensis JCM 14545 and Amycolatopsis magusensis DSM 45510, with 16S rRNA gene sequence similarities of 97.77, 97.20 and 97.19 %, respectively. Phylogenomic analysis based on the whole genome data supported that strain AU-G6 was distantly related to the Amycolatopsis species. The isolates shared a range of phenotypic markers typical of members of the genus Amycolatopsis, but also had a range of cultural, physiological and biochemical characteristics that separated them from related Amycolatopsis species. The isolates showed growth only in media supplemented with salt, indicating their marine origin. The cell wall of the isolates contained meso-diaminopimelic acid, and arabinose and galactose were detected as diagnostic sugars (type IV). The main menaquinone was MK-9(H4). The main polar lipids were phosphatidylethanolamine, hydroxy-phosphotidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol (type II). The fatty acid type was 3c. The combined genotypic and phenotypic data indicated that the two isolates represent a novel species of the genus Amycolatopsis. The name proposed for this species is Amycolatopsis antarctica sp. nov., with type strain AU-G6 (=CGMCC 4.7351=NBRC 112404).
从南极大型藻类泡囊腺囊藻（Adenocystis utricularis (Bory) Skottsberg）表面分离出两株中度嗜冷放线菌菌株，分别命名为AU-G6和AU-A3.2，并基于多相研究对其进行了分类学特征分析。这两株菌株具有几乎相同的16S rRNA基因序列，在假诺卡氏菌科的拟无枝酸菌属内形成了一个独特的进化分支。它们在系统发育上与黑色拟无枝酸菌JCM 14717、明尼苏达拟无枝酸菌JCM 14545和马古森拟无枝酸菌DSM 45510接近，16S rRNA基因序列相似性分别为97.77%、97.20%和97.19%。基于全基因组数据的系统基因组分析支持菌株AU-G6与拟无枝酸菌属的其他物种亲缘关系较远。这些分离株具有一系列拟无枝酸菌属成员典型的表型特征，但也具有一系列使其与相关拟无枝酸菌物种区分开来的培养、生理和生化特征。这些分离株仅在添加了盐的培养基中生长，表明它们起源于海洋。分离株的细胞壁含有内消旋二氨基庚二酸，检测到阿拉伯糖和半乳糖为诊断性糖类（IV型）。主要的甲基萘醌是MK-9(H4)。主要的极性脂质是磷脂酰乙醇胺、羟基磷脂酰乙醇胺、二磷脂酰甘油、磷脂酰甘油和磷脂酰肌醇（II型）。脂肪酸类型为3c。综合的基因型和表型数据表明，这两个分离株代表了拟无枝酸菌属的一个新物种。为此物种提议的名称是南极拟无枝酸菌（Amycolatopsis antarctica sp. nov.），模式菌株为AU-G6（=CGMCC 4.7,351=NBRC 112404）。
