Institute for Surgical Technology and Biomechanics, University of Bern, Stauffacherstrasse 78, 3014, Bern, Switzerland.
Department of Physical Chemistry, Eötvös Loránd University, Pázmány Péter Sétány 1/A, 1117, Budapest, Hungary.
Sci Rep. 2018 Jun 5;8(1):8601. doi: 10.1038/s41598-018-26836-0.
The interaction of osseous tissue with electric fields is an important subject. The electrical stimulation of bone promotes osteogenesis, while bone impedance has been proposed as a measure of osteoporosis, to follow fracture healing, or as a method to improve safety of surgical procedures. However, a deeper understanding of the electrical properties of bone and their relation to the architecture of osseous tissue is required to extend the range of use of electrical measurements to clinical studies. In this paper we apply electrical impedance spectroscopy to study the conductivity of fresh bovine tibia and we correlate the measured conductivities with its structural properties. Impedance was measured using a custom-made cell and a potentiostat. Bone conductivity was determined at 100 kHz, where the phase shift was negligible. A good agreement (R = 0.83) was found between the measured conductivity and the bone volume fraction, determined on microCT images. Based on this relationship, an equivalent circuit model was created for bone samples. The results of this ex-vivo study are comparable to previous in-vivo observations reporting bone resistivity as a function of bone density. This information can be used to construct a map of the tissue resistivity directly derived from clinical images.
骨组织与电场的相互作用是一个重要的研究课题。电刺激骨骼可以促进成骨，而骨阻抗被认为是骨质疏松症的一种测量方法，可以用于监测骨折愈合，或者作为提高手术安全性的一种方法。然而，为了将电测量的应用范围扩展到临床研究，需要更深入地了解骨的电学性质及其与骨组织结构的关系。在本文中，我们应用交流阻抗谱研究新鲜牛胫骨的电导率，并将测量的电导率与其结构特性相关联。使用定制的细胞和恒电位仪进行阻抗测量。在相位偏移可忽略不计的 100kHz 下确定骨的电导率。在微 CT 图像上确定的骨体积分数与测量的电导率之间存在良好的一致性（R=0.83）。基于这种关系，为骨样本创建了等效电路模型。这项离体研究的结果与之前报告的作为骨密度函数的骨电阻率的体内观察结果相吻合。该信息可用于直接从临床图像构建组织电阻率图。
