Yeargan M R, Allen G P, Bryans J T
J Clin Microbiol. 1985 May;21(5):694-7. doi: 10.1128/jcm.21.5.694-697.1985.
Two antigenically similar subtypes of equine herpesvirus 1 (EHV-1) cause disease in horses. A procedure for rapid differentiation of the two EHV-1 subtypes with monoclonal antibodies was developed. Subtype-specific pools of monoclonal antibodies were constructed, characterized, and used in enzyme immunofiltration and indirect immunofluorescence assays to subtype 50 epizootiologically unrelated field isolates of EHV-1. Both assays allowed accurate subtype identification of each EHV-1 isolate with the monoclonal antibody pools. The subtyping procedures were simple and amenable to typing many isolates at one time and permitted unambiguous EHV-1 subtype identification with 3 h after isolation of the virus in tissue culture.
马疱疹病毒1型(EHV-1)的两种抗原相似亚型可导致马匹发病。开发了一种使用单克隆抗体快速区分这两种EHV-1亚型的方法。构建、表征了亚型特异性单克隆抗体库,并将其用于酶免疫过滤和间接免疫荧光试验,以对50株流行病学上无关的EHV-1野外分离株进行亚型鉴定。两种试验都能通过单克隆抗体库准确鉴定每株EHV-1分离株的亚型。亚型鉴定程序简单,适合一次性对许多分离株进行分型,并且在病毒在组织培养中分离后3小时内即可明确鉴定EHV-1亚型。