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1
Rapid subtyping of equine herpesvirus 1 with monoclonal antibodies.利用单克隆抗体对马疱疹病毒1型进行快速分型
J Clin Microbiol. 1985 May;21(5):694-7. doi: 10.1128/jcm.21.5.694-697.1985.
2
Isolation and characterization of monoclonal antibodies against an attenuated vaccine strain of equine herpesvirus type 1 (EHV-1).抗马疱疹病毒1型（EHV-1）减毒疫苗株单克隆抗体的分离与鉴定
Vet Microbiol. 1988 Sep;18(1):95-101. doi: 10.1016/0378-1135(88)90119-8.
3
Rapid diagnosis and characterization of equid herpesvirus 1 using monoclonal antibodies.使用单克隆抗体对马疱疹病毒1型进行快速诊断和鉴定
Tierarztl Prax Suppl. 1987;2:37-40.
4
Epizootiological aspects of type 1 and type 4 equine herpesvirus infections among horse populations.马群中1型和4型马疱疹病毒感染的流行病学特征
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A type-specific conformational epitope on the nucleocapsid of equid herpesvirus-1 and its use in diagnosis.马疱疹病毒1型核衣壳上的型特异性构象表位及其在诊断中的应用。
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Equine herpesvirus 4 infected domestic horses associated with Sintashta spoke-wheeled chariots around 4,000 years ago.约4000年前，马疱疹病毒4感染了与辛塔什塔辐条轮战车相关的家养马匹。
Virus Evol. 2024 Jan 12;10(1):vead087. doi: 10.1093/ve/vead087. eCollection 2024.
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Equid herpesvirus 9 (EHV-9) isolates from zebras in Ontario, Canada, 1989 to 2007.1989年至2007年从加拿大安大略省斑马身上分离出的马疱疹病毒9型（EHV-9）毒株。
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Equine West Nile encephalitis, United States.美国马西尼罗河脑炎
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Equine herpesviruses 1 and 4: amplification and differentiation by polymerase chain reaction.马疱疹病毒1型和4型：通过聚合酶链反应进行扩增和鉴别
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9
Epitopes of glycoprotein G of equine herpesviruses 4 and 1 located near the C termini elicit type-specific antibody responses in the natural host.马疱疹病毒4型和1型糖蛋白G靠近C端的表位在天然宿主中引发型特异性抗体反应。
J Virol. 1993 Oct;67(10):6332-8. doi: 10.1128/JVI.67.10.6332-6338.1993.
10
A type-specific conformational epitope on the nucleocapsid of equid herpesvirus-1 and its use in diagnosis.马疱疹病毒1型核衣壳上的型特异性构象表位及其在诊断中的应用。
Arch Virol. 1993;132(1-2):133-44. doi: 10.1007/BF01309848.

本文引用的文献

1
Isolation of equine abortion virus from natural cases of equine abortion in horse kidney cell culture.
Jpn J Exp Med. 1959 Dec;29:643-9.
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Epizootiology of equine viral rhinopneumonitis.马病毒性鼻肺炎的流行病学
J Am Vet Med Assoc. 1963 Jan 1;142:31-7.
3
A planned infection program for immunizing mares against viral rhinopneumonitis.一项针对母马进行病毒性鼻肺炎免疫接种的计划感染方案。
Cornell Vet. 1963 Apr;53:249-62.
4
Molecular epizootiologic studies of equine herpesvirus-1 infections by restriction endonuclease fingerprinting of viral DNA.通过病毒DNA的限制性内切酶指纹图谱对马疱疹病毒1型感染进行分子流行病学研究。
Am J Vet Res. 1983 Feb;44(2):263-71.
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Typing of clinical herpes simplex virus isolates with mouse monoclonal antibodies to herpes simplex virus types 1 and 2: comparison with type-specific rabbit antisera and restriction endonuclease analysis of viral DNA.用抗1型和2型单纯疱疹病毒的小鼠单克隆抗体对临床单纯疱疹病毒分离株进行分型：与型特异性兔抗血清及病毒DNA限制性内切酶分析的比较
J Clin Microbiol. 1983 Jan;17(1):92-6. doi: 10.1128/jcm.17.1.92-96.1983.
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A rapid enzyme immunofiltration technique using monoclonal antibodies to serotype herpes simplex virus.一种使用针对单纯疱疹病毒血清型的单克隆抗体的快速酶免疫过滤技术。
J Med Virol. 1982;9(4):299-305. doi: 10.1002/jmv.1890090408.
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Identification and typing of herpes simplex viruses with monoclonal antibodies.用单克隆抗体对单纯疱疹病毒进行鉴定和分型
J Clin Microbiol. 1982 Jul;16(1):205-8. doi: 10.1128/jcm.16.1.205-208.1982.
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Serologic and molecular comparisons of several equine herpesvirus type 1 strains.几种1型马疱疹病毒毒株的血清学和分子比较
Am J Vet Res. 1981 Dec;42(12):2099-104.
9
Differentiation of respiratory and abortigenic isolates of equine herpesvirus 1 by restriction endonucleases.用限制性内切酶区分马疱疹病毒1型的呼吸道分离株和流产诱导分离株。
Science. 1981 Oct 30;214(4520):562-4. doi: 10.1126/science.6270790.
10
Differentiation of sub-types of equine herpesvirus I by restriction endonuclease analysis.通过限制性内切酶分析对马疱疹病毒I亚型进行鉴别
Aust Vet J. 1981 Mar;57(3):148-9. doi: 10.1111/j.1751-0813.1981.tb00495.x.

利用单克隆抗体对马疱疹病毒1型进行快速分型

Rapid subtyping of equine herpesvirus 1 with monoclonal antibodies.

作者信息

Yeargan M R, Allen G P, Bryans J T

出版信息

J Clin Microbiol. 1985 May;21(5):694-7. doi: 10.1128/jcm.21.5.694-697.1985.

DOI:10.1128/jcm.21.5.694-697.1985
PMID:2987299
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC271760/
Abstract

Two antigenically similar subtypes of equine herpesvirus 1 (EHV-1) cause disease in horses. A procedure for rapid differentiation of the two EHV-1 subtypes with monoclonal antibodies was developed. Subtype-specific pools of monoclonal antibodies were constructed, characterized, and used in enzyme immunofiltration and indirect immunofluorescence assays to subtype 50 epizootiologically unrelated field isolates of EHV-1. Both assays allowed accurate subtype identification of each EHV-1 isolate with the monoclonal antibody pools. The subtyping procedures were simple and amenable to typing many isolates at one time and permitted unambiguous EHV-1 subtype identification with 3 h after isolation of the virus in tissue culture.

摘要

马疱疹病毒1型（EHV-1）的两种抗原相似亚型可导致马匹发病。开发了一种使用单克隆抗体快速区分这两种EHV-1亚型的方法。构建、表征了亚型特异性单克隆抗体库，并将其用于酶免疫过滤和间接免疫荧光试验，以对50株流行病学上无关的EHV-1野外分离株进行亚型鉴定。两种试验都能通过单克隆抗体库准确鉴定每株EHV-1分离株的亚型。亚型鉴定程序简单，适合一次性对许多分离株进行分型，并且在病毒在组织培养中分离后3小时内即可明确鉴定EHV-1亚型。