Laboratorio de Neurociencias Moleculares e Integrativas, Escuela de Medicina, División Ciencias de la Salud, Universidad Anáhuac Mayab, Carretera Mérida-Progreso Km. 15.5, A.P. 96 Cordemex, C.P. 97310, Mérida, Yucatán, Mexico.
Intercontinental Neuroscience Research Group, Mérida, Yucatán, Mexico.
Neurochem Res. 2018 Aug;43(8):1511-1518. doi: 10.1007/s11064-018-2565-0. Epub 2018 Jun 6.
Cannabis sativa is a plant that contains more than 500 components, of which the most studied are Δ-tetrahydrocannabinol (Δ-THC) and cannabidiol (CBD). Several studies have indicated that CBD displays neurobiological effects, including wake promotion. Moreover, experimental evidence has shown that injections of CBD enhance wake-related compounds, such as monoamines (dopamine, serotonin, epinephrine, and norepinephrine). However, no clear evidence is available regarding the effects of CBD on additional wake-related neurochemicals such as acetylcholine (ACh). Here, we demonstrate that systemic injections of CBD (0, 5, 10 or 30 mg/kg, i.p.) at the beginning of the lights-on period, increase the extracellular levels of ACh collected from the basal forebrain and measured by microdialysis and HPLC means. Moreover, the time course effects on the contents of ACh were present 5 h post-injection of CBD. Altogether, these data demonstrate that CBD increases ACh levels in a brain region related to wake control. This study is the first to show the effects of ACh levels in CBD-treated rats and suggests that the basal forebrain might be a site of action of CBD for wakefulness modulation.
大麻是一种含有超过 500 种成分的植物，其中研究最多的是Δ-四氢大麻酚（Δ-THC）和大麻二酚（CBD）。多项研究表明，CBD 具有神经生物学效应，包括促进觉醒。此外，实验证据表明，CBD 注射可增强与觉醒相关的化合物，如单胺（多巴胺、血清素、肾上腺素和去甲肾上腺素）。然而，关于 CBD 对乙酰胆碱（ACh）等其他与觉醒相关的神经化学物质的影响，目前尚无明确证据。在这里，我们证明 CBD（0、5、10 或 30mg/kg，ip）在光照开始时系统注射可增加通过微透析和 HPLC 手段从基底前脑收集的 ACh 的细胞外水平。此外，CBD 注射后 5 小时即可观察到对 ACh 含量的时间过程影响。总的来说，这些数据表明 CBD 可增加与觉醒控制相关脑区的 ACh 水平。这项研究首次显示了 CBD 处理大鼠中 ACh 水平的影响，并表明基底前脑可能是 CBD 调节觉醒的作用部位。
