Limbach K J, Wu R
Nucleic Acids Res. 1985 Jan 25;13(2):631-44. doi: 10.1093/nar/13.2.631.
Analysis of total Drosophila melanogaster DNA by genomic blot hybridization indicates that two cytochrome c-like sequences exist in the Drosophila genome. These two sequences, DC3 and DC4, have been isolated from a Charon 4A-D. melanogaster genomic library. DC3 and DC4 are located within a 4 kb region of DNA, at position 36A 10-11, on the left arm of chromosome 2. The nucleotide sequence of these two clones has been determined. Both DC3 and DC4 can encode functional cytochrome c proteins. The polypeptide sequences predicted by these two genes, however, differ at 32 amino acid residues. DC4 is expressed at varying, but relatively high levels throughout Drosophila development. In contrast, DC3 is expressed at constant, but relatively low levels throughout development.
通过基因组印迹杂交对黑腹果蝇的总DNA进行分析表明，果蝇基因组中存在两个细胞色素c样序列。这两个序列，即DC3和DC4，已从Charon 4A-黑腹果蝇基因组文库中分离出来。DC3和DC4位于DNA的一个4 kb区域内，在2号染色体左臂的36A 10-11位置。这两个克隆的核苷酸序列已被确定。DC3和DC4都可以编码功能性细胞色素c蛋白。然而，这两个基因预测的多肽序列在32个氨基酸残基处存在差异。DC4在果蝇发育过程中表达水平不同，但相对较高。相比之下，DC3在整个发育过程中表达水平恒定，但相对较低。
