Gaut B S, Clegg M T
Department of Botany and Plant Sciences, University of California, Riverside 92520.
Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2060-4. doi: 10.1073/pnas.88.6.2060.
The molecular evolution of the alcohol dehydrogenase 1 (Adh1; alcohol:NAD+ oxidoreductase, EC 1.1.1.1) locus in members of the grass family is analyzed. We report the complete DNA sequence of a genomic clone of Adh1 from Pennisetum glaucum cv. Tift 23DB (pearl millet). The gene is characterized by ten exons and nine introns. The 5' flanking region of the gene contains sequences corresponding to the anaerobic regulatory element as well as sequences corresponding to the TATA box and the CAAT box identified in the maize Adh1-1S flanking regions. Exon sequences from Pennisetum and maize have been subjected to relative rate tests; the maize and Pennisetum Adh1 lineages evolve at equal rates. These results are compared with similar relative rate studies by using the chloroplast DNA encoding the ribulose-1,5-bisphosphate carboxylase (rbcL) gene. Evolutionary rates of Adh1 are estimated. Nonsynonymous rates are found to be 2.50 x 10(-10) substitutions per site per year, whereas synonymous rates are approximately 7.90 x 10(-9) substitutions per site per year. Molecular phylogenies of the Poaceae based upon Adh1 data are presented.
分析了禾本科植物中乙醇脱氢酶1（Adh1；乙醇：NAD+氧化还原酶，EC 1.1.1.1）基因座的分子进化。我们报道了来自黍属品种提夫特23DB（珍珠粟）的Adh1基因组克隆的完整DNA序列。该基因由10个外显子和9个内含子组成。基因的5'侧翼区域包含与厌氧调控元件相对应的序列，以及与在玉米Adh1-1S侧翼区域中鉴定出的TATA盒和CAAT盒相对应的序列。对黍属和玉米的外显子序列进行了相对速率测试；玉米和黍属的Adh1谱系以相同的速率进化。通过使用编码核酮糖-1,5-二磷酸羧化酶（rbcL）基因的叶绿体DNA，将这些结果与类似的相对速率研究进行了比较。估计了Adh1的进化速率。发现非同义速率为每年每个位点2.50×10^(-10)个替换，而同义速率约为每年每个位点7.90×10^(-9)个替换。给出了基于Adh1数据的禾本科分子系统发育树。
