Nhatrang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, 02 Hung Vuong Street, Nhatrang, Viet Nam.
Nhatrang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, 02 Hung Vuong Street, Nhatrang, Viet Nam.
Int J Biol Macromol. 2018 Oct 1;117:1101-1109. doi: 10.1016/j.ijbiomac.2018.06.017. Epub 2018 Jun 6.
In the present study, three sulfated polysaccharides, two fractions of fucosylated chondroitin sulfates, and one sulfated fucan were isolated from the body wall of the Vietnamese sea cucumber Stichopus variegatus. The structure of the sulfated fucan fraction SvF3 from S. variegatus was investigated for the first time. According to NMR spectroscopy data, the sulfated fucan SvF3 contained 1,2- and 1,3-linked α-l-fucopyranose residues. Sulfate groups were found at the 2 and/or 4 positions. The structural analysis of fucoidan was assisted by tandem mass spectrometry; the recently-developed technique of autohydrolysis in heavy‑oxygen water for the obtaining of selectively labeled fucoidan fragments was applied. The labeling (+2 Da mass shift at the reducing end) allowed us to assign MS/MS data unambiguously, and thus to confirm the NMR data and revealed minor sulfation at position 3. It was shown that the sulfated fucan SvF3 was not cytotoxic to human breast cancer T-47D and MDA-MB-231 cell lines, and it inhibited colony formation of those cells in vitro. SvF3 also possessed slight activity against migration of MDA-MB-231 cells in vitro.
在本研究中，从越南海参体壁中分离出三种硫酸化多糖、两种岩藻糖基硫酸软骨素和一种硫酸化岩藻聚糖。首次对来自海参的硫酸化岩藻聚糖 SvF3 的结构进行了研究。根据 NMR 光谱数据，硫酸化岩藻聚糖 SvF3 含有 1,2-和 1,3-连接的α-l-岩藻糖吡喃糖基。硫酸基位于 2 和/或 4 位。通过串联质谱法辅助褐藻糖胶的结构分析；应用了新开发的重氧水自动水解技术来获得选择性标记的褐藻糖胶片段。标记（还原端增加 2 Da 质量位移）使我们能够明确地分配 MS/MS 数据，从而确认 NMR 数据并揭示 3 位的轻微磺化。结果表明，硫酸化岩藻聚糖 SvF3 对人乳腺癌 T-47D 和 MDA-MB-231 细胞系无细胞毒性，并抑制这些细胞的体外集落形成。SvF3 对 MDA-MB-231 细胞的体外迁移也有轻微的抑制作用。
