Benslima Abdelkarim, Sellimi Sabrine, Hamdi Marwa, Nasri Rim, Jridi Mourad, Cot Didier, Li Suming, Nasri Moncef, Zouari Nacim
Laboratory of Enzyme Engineering and Microbiology National Engineering School of Sfax (ENIS) University of Sfax Sfax Tunisia.
Higher Institute of Applied Biology of Medenine (ISBAM) University of Gabes Medenine Tunisia.
Food Sci Nutr. 2021 Jan 16;9(3):1551-1563. doi: 10.1002/fsn3.2130. eCollection 2021 Mar.
A fucoidan, sulfated polysaccharide, was extracted from the brown seaweed during 4 harvest periods (December, April, July, and September) and studied for its structural and chemical properties. The fucoidan (CSF) showed important variation in sulfate content ranging from 7.8% in December to 34.8% in July. This was confirmed by Fourier transform infrared and nuclear magnetic resonance spectroscopies showing characteristic signals of sulfated polysaccharides. Molecular mass of the CSF varied as a function of season from 3,745 in December to 26,390 Da in July. Gas chromatography-mass spectroscopy showed that CSF fractions were "mannogalactofucans" composed mainly of mannose, fucose, and galactose with low levels of other monosaccharides. Moreover, interesting in vitro antioxidant activities that depend on the harvest season were noted for CSF. Thus, the present work might contribute to establish criteria for extracting bioactive fucoidans from an endemic Tunisian seaweed .
从褐藻中提取了一种岩藻依聚糖（一种硫酸化多糖），该褐藻采集于4个收获期（12月、4月、7月和9月），并对其结构和化学性质进行了研究。岩藻依聚糖（CSF）的硫酸盐含量显示出重要变化，范围从12月的7.8%到7月的34.8%。傅里叶变换红外光谱和核磁共振光谱证实了这一点，显示出硫酸化多糖的特征信号。CSF的分子量随季节变化，从12月的3745到7月的26390道尔顿。气相色谱 - 质谱分析表明，CSF组分是“甘露半乳岩藻聚糖”，主要由甘露糖、岩藻糖和半乳糖组成，其他单糖含量较低。此外，还注意到CSF具有取决于收获季节的有趣体外抗氧化活性。因此，本研究可能有助于建立从突尼斯本土海藻中提取生物活性岩藻依聚糖的标准。
