Wang Qiqi, Tang Guanghui
Key Laboratory of State Forestry Administration on Management of Western Forest Bio-Disaster, College of Forestry, Northwest A&F University, Yangling, Shaanxi, China.
Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
PeerJ. 2018 Jun 5;6:e4919. doi: 10.7717/peerj.4919. eCollection 2018.
In this study, the mitochondrial genomes (mitogenomes) of two walnut leaf insect pests, and . , were sequenced by Sanger sequencing technology. The mitogenome of . was complete at 16,109 bp in length, while the mitogenome of . (14,277 bp) was partial. The genomic analyses indicated that both mitogenomes have the typical gene content and arrangement. The formerly identified elements, 'TAGTA' between () and , and 'ATGATAA' between and , were more conserved than that between and , which was 'ATGTTAA' in Coleoptera excluding Polyphaga. Phylogenetic analyses of the 13 protein-coding genes from 36 coleopteran species well supported a close affinity between the subfamily Chrysomelinae including . and . and Galerucinae, as well as a sister relationship of ((Eumolpinae + Cryptocephalinae) + Cassidinae) within Chrysomelidae.
在本研究中，采用桑格测序技术对两种核桃叶害虫和的线粒体基因组（mitogenomes）进行了测序。的线粒体基因组长度为16,109 bp，是完整的，而的线粒体基因组（14,277 bp）是部分的。基因组分析表明，两个线粒体基因组都具有典型的基因组成和排列。先前鉴定的元件，在（）和之间的“TAGTA”，以及在和之间的“ATGATAA”，比在和之间的元件更保守，后者在除多食亚目外的鞘翅目中是“ATGTTAA”。对36种鞘翅目物种的13个蛋白质编码基因进行的系统发育分析有力地支持了包括和在内的叶甲亚科与叶甲亚科之间的密切亲缘关系，以及叶甲科内（（真叶甲亚科+隐头叶甲亚科）+龟甲亚科）的姐妹关系。
