Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China.
College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Biomed Res Int. 2018 May 20;2018:4149707. doi: 10.1155/2018/4149707. eCollection 2018.
Lung cancer is one of the leading causes of cancer-related death in the world. MicroRNA- (miR-) 628-3p plays critical roles in many cancers, including lung cancer. We investigated how miR-628-3p affected migration and apoptosis in A549 cells. We used bioinformatics algorithms to predict the miR-628-3p target gene to study the molecular mechanism by which miR-628-3p contributes to lung cancer. Then, we used the luciferase reporter assay to identify whether heat shock protein 90a is a direct target of miR-628-3p. Western blotting and quantitative real-time PCR showed that miR-628-3p downregulated HSP90a protein expression via a posttranscriptional mechanism. We confirm that miR-628-3p promotes apoptosis and inhibits migration in A549 cells by negatively regulating . Our results may reveal a novel strategy for lung cancer treatment.
肺癌是世界上癌症相关死亡的主要原因之一。microRNA- (miR-) 628-3p 在许多癌症中发挥着关键作用，包括肺癌。我们研究了 miR-628-3p 如何影响 A549 细胞的迁移和凋亡。我们使用生物信息学算法预测了 miR-628-3p 的靶基因，以研究 miR-628-3p 促进肺癌的分子机制。然后，我们使用荧光素酶报告基因检测来确定热休克蛋白 90a 是否是 miR-628-3p 的直接靶基因。Western blot 和实时定量 PCR 显示，miR-628-3p 通过转录后机制下调 HSP90a 蛋白表达。我们证实，miR-628-3p 通过负调控 HSP90a 促进 A549 细胞凋亡和抑制迁移。我们的结果可能揭示了一种治疗肺癌的新策略。
