Gallo-Payet N, Escher E
Endocrinology. 1985 Jul;117(1):38-46. doi: 10.1210/endo-117-1-38.
The results presented here demonstrate, for the first time, the presence of ACTH receptors in the zona glomerulosa of adrenal glands. We obtained the surprising result that the glomerulosa cells carry a higher concentration of ACTH receptors than the fasciculata cells. The analog [Phe2,Nle4]ACTH was iodinated by the iodogen method and separated by HPLC; it was obtained carrier-free and has a specific activity of 600 muCi/micrograms, retaining full biological potency. After 30 min of incubation at 22 C for concentrations of 2 X 10(-11) M [125I]ACTH, specific binding values were 4.85 +/- 0.44% (n = 15) and 1.85 +/- 0.18% (n = 15), respectively, for 50,000 glomerulosa or fasciculata cells. For the glomerulosa, our results indicated a density of 6.5 X 10(4), receptors/cell of the high affinity type (Kd1 = 7.6 X 10(-11) M) and 1.0 X 10(6) receptors of the low affinity type (Kd2 = 1.2 X 10(-9) M). In the zona fasciculata, we found 7.2 X 10(3) receptors of high affinity (Kd1 = 1.1 X 10(-11) M) per cell and 6.3 X 10(5) of low affinity (Kd2 = 2.9 X 10(-9) M). The dissociation constant for the high affinity site of the glomerulosa cells was in excellent correlation with the half-maximal stimulation dose of ACTH for aldosterone and corticosterone (Kd1 = 7.6 X 10(-11) M vs. ED50 of 8 X 10(-11) and 3 X 10(-11) M). Results from primary cultures showed a decrease in binding capacity after 1 day in culture and then an increase to the initial value after 3 days in culture.
此处展示的结果首次证明了肾上腺球状带中存在促肾上腺皮质激素（ACTH）受体。我们获得了一个惊人的结果，即球状带细胞携带的ACTH受体浓度高于束状带细胞。类似物[苯丙氨酸2，亮氨酸4]ACTH通过碘代法碘化并通过高效液相色谱（HPLC）分离；得到了无载体的产物，其比活为600μCi/μg，保留了全部生物活性。在22℃下孵育30分钟后，对于浓度为2×10⁻¹¹M的[¹²⁵I]ACTH，50,000个球状带或束状带细胞的特异性结合值分别为4.85±0.44%（n = 15）和1.85±0.18%（n = 15）。对于球状带，我们的结果表明高亲和力类型（Kd1 = 7.6×10⁻¹¹M）的受体密度为6.5×10⁴个/细胞，低亲和力类型（Kd2 = 1.2×10⁻⁹M）的受体为1.0×10⁶个。在束状带中，我们发现每个细胞有7.2×10³个高亲和力（Kd1 = 1.1×10⁻¹¹M）受体和6.3×10⁵个低亲和力（Kd2 = 2.9×10⁻⁹M）受体。球状带细胞高亲和力位点的解离常数与ACTH对醛固酮和皮质酮的半数最大刺激剂量具有极好的相关性（Kd1 = 7.6×10⁻¹¹M，而半数有效剂量分别为8×10⁻¹¹M和³×10⁻¹¹M）。原代培养结果显示，培养1天后结合能力下降，然后在培养3天后增加到初始值。
