Brazilian Nanotechnology National Laboratory (LNNano) , Brazilian Center for Research in Energy and Materials (CNPEM) , 13083-970 Campinas , Sao Paulo , Brazil.
ACS Appl Mater Interfaces. 2018 Jul 5;10(26):21891-21900. doi: 10.1021/acsami.8b06149. Epub 2018 Jun 22.
A new nanohydrogel drug delivery platform based on Laponite nanodiscs, polyacrylate, and sodium phosphate salts is described. The hybrid nanohydrogel is tailored to obtain soft and flexible nanohydrogels with G' around 3 kPa, which has been proposed as the ideal stiffness for drug delivery applications. In vitro studies demonstrate that the new nanohydrogels are biocompatible, biodegradable, nonswellable, pH-responsive, and noncytotoxic and are able to deliver antineoplastic drugs into cancer cells. The IC of nanohydrogels containing cisplatin, 4-fluorouracil, and cyclophosphamide is significantly lower than the IC of the free drugs. In vivo experiments suggest that the new nanomaterials are biocompatible and do not accumulate in crucial organs. The simple formulation procedure enables encapsulation of virtually any water-soluble molecule, without the need for chemical modification of the guests. These nanohydrogels are a versatile platform that enables the simultaneous encapsulation of several cancer drugs, yielding an efficient drug cocktail delivery system, which for instance presents a positive synergistic effect against MCF-7 cells.
一种基于 Laponite 纳米盘、聚丙烯酸和磷酸钠盐的新型纳米水凝胶药物输送平台。该混合纳米水凝胶经过精心设计，可获得 G'约为 3 kPa 的柔软、灵活的纳米水凝胶，这被认为是药物输送应用的理想刚度。体外研究表明，新型纳米水凝胶具有生物相容性、可生物降解、不可膨胀、pH 响应性和非细胞毒性，能够将抗肿瘤药物递送到癌细胞中。含有顺铂、5-氟尿嘧啶和环磷酰胺的纳米水凝胶的 IC 明显低于游离药物的 IC。体内实验表明，新型纳米材料具有生物相容性，不会在关键器官中积累。简单的配方程序可实现几乎任何水溶性分子的封装，而无需对客体进行化学修饰。这些纳米水凝胶是一种多功能平台，可同时封装几种癌症药物，形成有效的药物鸡尾酒输送系统，例如，对 MCF-7 细胞具有积极的协同作用。
