Key Laboratory of Chinese Materia Medica, Ministry of Education, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
Molecules. 2018 Jun 11;23(6):1412. doi: 10.3390/molecules23061412.
Hance has been used in fractures for thousands of years, but research on its active components, such as morroniside, until now had not been carried out. In this study, morroniside was taken as the leading compound, and fourteen derivatives were synthesized. The promotion of osteoblast proliferation effect of the derivatives was evaluated on MC3T3-E1 cells. Five derivatives (, , , , and ) showed a good proliferation effect on MC3T3-E1 cells, and their promoted expression effects on OC (Osteocalcin) and ALP (Alkaline phosphatase) in MC3T3-E1 cells were measured. Compound was shown to have the strongest proliferation effect (EC = 14.78 ± 1.17 μg/mL) and to significantly promote the expression of OC and ALP.
汉防己甲素在骨折治疗中已经使用了数千年，但对其活性成分（如汉防己甲素）的研究直到现在还没有进行。在这项研究中，汉防己甲素被作为主导化合物，合成了 14 种衍生物。在 MC3T3-E1 细胞上评估了这些衍生物促进成骨细胞增殖的效果。其中 5 种衍生物（、、、、和）对 MC3T3-E1 细胞表现出良好的增殖效果，并测量了它们对 MC3T3-E1 细胞中 OC（骨钙素）和 ALP（碱性磷酸酶）的促进表达效果。化合物 显示出最强的增殖效果（EC = 14.78 ± 1.17 μg/mL），并能显著促进 OC 和 ALP 的表达。
