Badescu Laurentiu, Badulescu Oana, Badescu Magda, Ciocoiu Manuela
Department of Cellular and Molecular Biology, University of Medicine and Pharmacy "Grigore T. Popa" Iasi, 16 Universitatii Street, 700115 Iasi, Romania.
Evid Based Complement Alternat Med. 2012;2012:848269. doi: 10.1155/2012/848269. Epub 2012 Sep 13.
The effects of polyphenols extracted from Sambucus nigra fruit were studied in streptozotocin- (STZ-) induced hyperglycemic rats to evaluate its possible antioxidant, anti-inflammatory, antiglycosylation activity, and antiosteoporosis effects in diabetes. DEXA bone mineral density tests were performed in order to determine bone mineral density (BMD), bone mineral content (BMC), and fat (%Fat) in control and diabetic animals, before and after polyphenol delivery. As compared to the normoglycemic group, the rats treated with STZ (60 mg/kg body weight) revealed a significant malondialdehyde (MDA) increase, as an index of the lipid peroxidation level, by 69%, while the total antioxidant activity (TAS) dropped by 36%, with a consistently significant decrease (P < 0.05) in the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPX). Also, the treatment of rats with STZ revealed a significant increase of IL-6, glycosylated haemoglobin (HbA(1c)), and osteopenia detected by DEXA bone mineral density tests. The recorded results highlight a significant improvement (P < 0.001) in the antioxidative capacity of the serum in diabetic rats treated with natural polyphenols, bringing back to normal the concentration of reduced glutathione (GSH), as well as an important decrease in the serum concentration of MDA, with improved osteoporosis status. Knowing the effects of polyphenols could lead to the use of the polyphenolic extract of Sambucus nigra as a dietary supplement in diabetic osteoporosis.
研究了黑接骨木果实中提取的多酚对链脲佐菌素（STZ）诱导的高血糖大鼠的影响，以评估其在糖尿病中可能的抗氧化、抗炎、抗糖基化活性和抗骨质疏松作用。在给予多酚前后，对对照组和糖尿病动物进行双能X线吸收法（DEXA）骨密度测试，以确定骨密度（BMD）、骨矿物质含量（BMC）和脂肪含量（%Fat）。与正常血糖组相比，用STZ（60mg/kg体重）处理的大鼠脂质过氧化水平指标丙二醛（MDA）显著增加69%，而总抗氧化活性（TAS）下降36%，超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPX）活性持续显著降低（P<0.05）。此外，用STZ处理大鼠还显示白细胞介素-6、糖化血红蛋白（HbA(1c)）显著增加，DEXA骨密度测试检测到骨质减少。记录结果表明，用天然多酚处理的糖尿病大鼠血清抗氧化能力显著提高（P<0.001），还原型谷胱甘肽（GSH）浓度恢复正常，血清MDA浓度显著降低，骨质疏松状况改善。了解多酚的作用可能会促使将黑接骨木的多酚提取物用作糖尿病骨质疏松症的膳食补充剂。
