Chen Yu-Pei, Liang Chia-Hua, Wu Hong-Tan, Pang Hai-Yue, Chen Chuan, Wang Guey-Horng, Chan Leong-Perng
Research Center of Natural Cosmeceuticals Engineering, Xiamen Medical College, Xiamen, 361023 Fujian China.
Application Technique Engineering Center of Natural Cosmeceuticals, College of Fuijan Province, Xiamen Medical College, Xiamen, 361023 Fujian China.
J Food Sci Technol. 2018 Jun;55(6):2310-2317. doi: 10.1007/s13197-018-3148-4. Epub 2018 May 3.
Milkfish (), which is resistant to water quality changes is the fourth largest aquaculture commodity. Abandoned wastes of fish scale and bones aggravate environmental pollution. In this study, the effect of collagen peptides isolated from milkfish scales (MSCP) by pepsin-soluble collagen method on cell viability was investigated. The antioxidant, anti-inflammatory, and DNA-protective activities of MSCP were also evaluated. Results revealed that more than 95% of viable cells were retained in human keratinocytes after addition of 100 mg/mL MSCP. Measurement of DPPH· and ABTS· + radical scavenging activities and cellular reactive oxygen species revealed the high antioxidant activities of MSCP. MSCP demonstrated anti-inflammatory activities by reducing lipoxygenase activity and nitric oxide (NO·) radicals. Moreover, DNA electrophoresis assay indicated that MSCP treatment can directly protect against cyclobutane di-pyrimidine production and DNA single-strand breaks, which are harmful effects of UV radiation and HO. Given its antioxidant, anti-inflammatory, and DNA-protective activities, MSCP has potential applications in cosmeceuticals and supplementary health food.
虱目鱼（）耐水质变化，是第四大水产养殖品种。鱼鳞和鱼骨等废弃废物加剧了环境污染。在本研究中，研究了通过胃蛋白酶可溶胶原蛋白法从虱目鱼鳞中分离得到的胶原蛋白肽（MSCP）对细胞活力的影响。还评估了MSCP的抗氧化、抗炎和DNA保护活性。结果显示，添加100mg/mL MSCP后，超过95%的活细胞保留在人角质形成细胞中。对DPPH·和ABTS·+自由基清除活性以及细胞活性氧的测定表明MSCP具有高抗氧化活性。MSCP通过降低脂氧合酶活性和一氧化氮（NO·）自由基表现出抗炎活性。此外，DNA电泳分析表明，MSCP处理可直接防止环丁烷二嘧啶的产生和DNA单链断裂，这是紫外线辐射和羟基自由基的有害影响。鉴于其抗氧化、抗炎和DNA保护活性，MSCP在药妆品和补充性健康食品中具有潜在应用。
