人巨细胞病毒抗体快速中和试验
Rapid neutralization assay for human cytomegalovirus antibody.
作者信息
Zerbini M, Plazzi M, Musiani M
出版信息
J Clin Microbiol. 1985 Jun;21(6):969-71. doi: 10.1128/jcm.21.6.969-971.1985.
Abstract
Human cytomegalovirus induces the appearance of immediate early antigens in infected cells 1 h after infection. This provided the basis for the development of a rapid neutralization assay for cytomegalovirus antibody which was able to yield results within a single day. Indirect immunofluorescence to visualize immediate early antigen-positive cells was applied to the rapid determination of cytomegalovirus-neutralizing antibody. The neutralization titers obtained with this assay on 92 serum samples were in accordance with the immune status as determined by enzyme-linked immunosorbent assay to cytomegalovirus-induced immediate early, early, and late antigens.
摘要
人巨细胞病毒在感染后1小时即可诱导被感染细胞出现即刻早期抗原。这为开发一种快速的巨细胞病毒抗体中和试验奠定了基础，该试验能够在一天内得出结果。将用于观察即刻早期抗原阳性细胞的间接免疫荧光法应用于巨细胞病毒中和抗体的快速检测。用该试验对92份血清样本测得的中和效价与通过酶联免疫吸附试验检测巨细胞病毒诱导的即刻早期、早期和晚期抗原所确定的免疫状态一致。
相似文献
1
Rapid neutralization assay for human cytomegalovirus antibody.人巨细胞病毒抗体快速中和试验
J Clin Microbiol. 1985 Jun;21(6):969-71. doi: 10.1128/jcm.21.6.969-971.1985.
2
Rapid detection of antibodies against cytomegalovirus induced immediate early and early antigens by an enzyme linked immunosorbent assay.通过酶联免疫吸附试验快速检测抗巨细胞病毒诱导的即刻早期和早期抗原的抗体。
J Clin Pathol. 1984 Feb;37(2):122-5. doi: 10.1136/jcp.37.2.122.
3
Rapid quantitation of cytomegalovirus and assay of neutralizing antibody by using monoclonal antibody to the major immediate-early viral protein.利用针对病毒主要立即早期蛋白的单克隆抗体对巨细胞病毒进行快速定量及中和抗体检测。
J Clin Microbiol. 1988 Mar;26(3):504-7. doi: 10.1128/jcm.26.3.504-507.1988.
4
Comparison of occurrence of antibodies to human cytomegalovirus as demonstrated by immunofluorescence and indirect hemagglutination techniques.通过免疫荧光和间接血凝技术对人巨细胞病毒抗体出现情况的比较。
J Clin Microbiol. 1979 Jan;9(1):149-51. doi: 10.1128/jcm.9.1.149-151.1979.
5
Antibodies to cytomegalovirus-induced pre-early nuclear antigen in the anticomplement-immunofluorescent test in comparison to IgG and IgM antibodies in the indirect and direct enzyme-linked immunosorbent assay in diagnosing cytomegalovirus infections.在诊断巨细胞病毒感染时，与间接和直接酶联免疫吸附测定中的IgG和IgM抗体相比，抗补体免疫荧光试验中针对巨细胞病毒诱导的早期核前抗原的抗体。
Arch Virol. 1984;80(4):305-13. doi: 10.1007/BF01311221.
6
Direct enzyme-linked immunosorbent assay that uses peroxidase-labeled antigen for determination of immunoglobulin M antibody to cytomegalovirus.使用过氧化物酶标记抗原的直接酶联免疫吸附测定法，用于检测巨细胞病毒免疫球蛋白M抗体。
J Clin Microbiol. 1981 Mar;13(3):416-22. doi: 10.1128/jcm.13.3.416-422.1981.
7
Humoral immune response to human cytomegalovirus infection: diagnostic potential of immunoglobulin class and IgG subclass antibody response to human cytomegalovirus early and late antigens.对人巨细胞病毒感染的体液免疫反应：免疫球蛋白类别及IgG亚类抗体对人巨细胞病毒早期和晚期抗原反应的诊断潜力
Clin Investig. 1993 Apr;71(4):270-6. doi: 10.1007/BF00184725.
8
Detection of immunoglobulin M and G antibodies against cytomegalovirus early and late antigens by enzyme-linked immunosorbent assay.通过酶联免疫吸附测定法检测针对巨细胞病毒早期和晚期抗原的免疫球蛋白M和G抗体。
J Clin Microbiol. 1984 Oct;20(4):763-71. doi: 10.1128/jcm.20.4.763-771.1984.
9
Detection of antibody to murine cytomegalovirus by enzyme-linked immunosorbent and indirect immunofluorescence assays.通过酶联免疫吸附试验和间接免疫荧光试验检测抗鼠巨细胞病毒抗体。
J Clin Microbiol. 1987 Apr;25(4):600-4. doi: 10.1128/jcm.25.4.600-604.1987.
10
Cytomegalovirus antibodies in renal transplant recipients.肾移植受者中的巨细胞病毒抗体
Acta Microbiol Pol. 1982;31(3-4):279-85.
引用本文的文献
1
Rapid quantitation of cytomegalovirus and assay of neutralizing antibody by using monoclonal antibody to the major immediate-early viral protein.利用针对病毒主要立即早期蛋白的单克隆抗体对巨细胞病毒进行快速定量及中和抗体检测。
J Clin Microbiol. 1988 Mar;26(3):504-7. doi: 10.1128/jcm.26.3.504-507.1988.
2
Dot immunoperoxidase assay using monoclonal antibody for direct detection of cytomegalovirus in urine samples.使用单克隆抗体的斑点免疫过氧化物酶测定法直接检测尿液样本中的巨细胞病毒。
J Clin Microbiol. 1987 Nov;25(11):2197-9. doi: 10.1128/jcm.25.11.2197-2199.1987.
本文引用的文献
1
A PLAQUE REDUCTION NEUTRALIZATION TEST FOR HUMAN CYTOMEGALOVIRUS.人巨细胞病毒蚀斑减少中和试验
Proc Soc Exp Biol Med. 1964 Oct;117:145-50. doi: 10.3181/00379727-117-29520.
2
Rapid detection of antibodies against cytomegalovirus induced immediate early and early antigens by an enzyme linked immunosorbent assay.通过酶联免疫吸附试验快速检测抗巨细胞病毒诱导的即刻早期和早期抗原的抗体。
J Clin Pathol. 1984 Feb;37(2):122-5. doi: 10.1136/jcp.37.2.122.
3
Influence of cell cycle on the efficiency of transfection with purified human cytomegalovirus DNA.
Arch Virol. 1983;78(3-4):287-92. doi: 10.1007/BF01311323.
4
Productive and abortive replication of human cytomegalovirus at different environmental pH values.人巨细胞病毒在不同环境pH值下的增殖性和流产性复制
Arch Virol. 1982;72(1-2):127-31. doi: 10.1007/BF01314457.
5
Microneutalization of cytomegalovirus.巨细胞病毒的微中和作用
J Infect Dis. 1980 Jul;142(1):102-5. doi: 10.1093/infdis/142.1.102.
6
7
Monoclonal antibodies to human cytomegalovirus: three surface membrane proteins with unique immunological and electrophoretic properties specify cross-reactive determinants.抗人巨细胞病毒单克隆抗体：三种具有独特免疫和电泳特性的表面膜蛋白可确定交叉反应决定簇。
Infect Immun. 1982 Jun;36(3):924-32. doi: 10.1128/iai.36.3.924-932.1982.
8
Serological screening for the prevention of transfusion-acquired cytomegalovirus infection.
J Infect. 1984 Sep;9(2):148-52. doi: 10.1016/s0163-4453(84)91078-8.
9
Three-day assay for human cytomegalovirus applicable to serum neutralization tests.适用于血清中和试验的人巨细胞病毒三日检测法。
Appl Microbiol. 1973 Jan;25(1):37-9. doi: 10.1128/am.25.1.37-39.1973.
10
DNA of human cytomegalovirus: size heterogeneity and defectiveness resulting from serial undiluted passage.人巨细胞病毒的DNA：连续未稀释传代导致的大小异质性和缺陷性
J Virol. 1979 Jul;31(1):231-9. doi: 10.1128/JVI.31.1.231-239.1979.
Zotero 插件