Influence of cell cycle on the efficiency of transfection with purified human cytomegalovirus DNA.

The efficiency of transfection of purified HCMV genome was studied in synchronized human embryo fibroblasts. The data show that HCMV DNA infection performed in S phase is more efficient than in cells infected in G2, M and G1 phases of cell cycle, respectively. These findings stress the importance of the metabolic state of cell cultures in HCMV genome transfection.