通过酶联免疫吸附试验和间接免疫荧光试验检测抗鼠巨细胞病毒抗体。
Detection of antibody to murine cytomegalovirus by enzyme-linked immunosorbent and indirect immunofluorescence assays.
作者信息
Classen D C, Morningstar J M, Shanley J D
出版信息
J Clin Microbiol. 1987 Apr;25(4):600-4. doi: 10.1128/jcm.25.4.600-604.1987.
Abstract
We have compared murine cytomegalovirus (MCMV) antibody determination by enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence assay. A comparison of antibody detection with 146 serum samples at a 1:20 dilution showed 100% agreement (60 negatives and 86 positives) between the assays. There was close agreement of endpoint determinations of sera by both methods. After experimental MCMV infection, antibody to MCMV was detected by both assays as early as day 7, and high titers persisted as late as 6 months. In contrast to immunocompetent littermates, athymic nude mice did not develop antibody after infection. Mice lacking antibody detectable by ELISA were susceptible to lethal MCMV challenge. In a survey of animals from five commercial sources, MCMV antibody was not detected unless mice were experimentally infected. MCMV antibody determination by ELISA is a convenient method, comparable to the indirect immunofluorescence assay in sensitivity and specificity.
摘要
我们比较了通过酶联免疫吸附测定（ELISA）和间接免疫荧光测定来检测小鼠巨细胞病毒（MCMV）抗体。对146份稀释度为1:20的血清样本进行抗体检测的比较显示，两种检测方法之间的一致性为100%（60份阴性和86份阳性）。两种方法对血清终点测定的结果非常一致。在实验性MCMV感染后，两种检测方法早在第7天就检测到了抗MCMV抗体，高滴度抗体一直持续到6个月。与具有免疫能力的同窝小鼠相比，无胸腺裸鼠在感染后未产生抗体。ELISA检测不到抗体的小鼠对致死性MCMV攻击敏感。在对来自五个商业来源的动物进行的调查中，除非小鼠经过实验性感染，否则检测不到MCMV抗体。通过ELISA测定MCMV抗体是一种简便的方法，在敏感性和特异性方面与间接免疫荧光测定相当。
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