Musiani M, Carpi C, Zerbini M
J Clin Pathol. 1984 Feb;37(2):122-5. doi: 10.1136/jcp.37.2.122.
An enzyme linked immunosorbent assay (ELISA) for detecting antibodies against cytomegalovirus induced immediate early antigens and early antigens was developed using purified nuclear antigens and was compared with the indirect immunofluorescence test. The tests were comparable in their ability to detect positive and negative sera, and antibody titres determined by both assays were similar. The use of ELISA for the detection of antibodies against cytomegalovirus induced immediate early and early antigens is advocated in diagnostic and research laboratories.
利用纯化的核抗原开发了一种用于检测抗巨细胞病毒诱导即刻早期抗原和早期抗原抗体的酶联免疫吸附测定(ELISA),并与间接免疫荧光试验进行了比较。两种试验在检测阳性和阴性血清的能力方面具有可比性,且两种测定法所确定的抗体滴度相似。在诊断和研究实验室中提倡使用ELISA检测抗巨细胞病毒诱导即刻早期和早期抗原的抗体。