Department of Pathology, University Hospitals Cleveland Medical Center, 44106 Cleveland, OH.
Department of Pathology, University Hospitals Cleveland Medical Center, 44106 Cleveland, OH.
Hum Pathol. 2018 Oct;80:163-169. doi: 10.1016/j.humpath.2018.06.007. Epub 2018 Jun 9.
The transcription factor SOX10 mediates the differentiation of neural crest-derived cells, and SOX10 by immunohistochemistry (IHC) is used primarily for the diagnosis of melanoma. SOX10 expression has been previously documented in benign breast myoepithelial cells. However there is limited literature on its expression in triple-negative breast carcinoma (TNBC). The aim was to study the clinical, pathologic and molecular profiles of SOX10+ tumors in TNBC. Tissue microarrays of TNBC were evaluated for SOX10 expression in 48 cases. SOX10 expression was correlated with clinical and pathologic features such as age, grade, and stage. Gene expression was analyzed on RNA extracted from formalin-fixed paraffin-embedded (FFPE) specimens with Affymetrix 2.0 HTA. Co-expression of SOX10 with androgen receptor (AR), WT1, gross cystic disease fluid protein-15 (GCDFP-15), mammaglobin, epidermal growth factor receptor (EGFR), CK5/6 and GATA transcription factor 3 (GATA3) were also assessed. The mean age was 59.38 (range, 28-90 years). Overall, 37.5% cases (18/48) were SOX10+. There was no association between SOX10 expression and age, grade or stage of patients; 6 of 10 (60%) cases of basal-like 1 (BL1), and 5 of 8 cases of unstable (UNS) molecular subtype were SOX10+. One of 5 basal-like-2 (BL2), 1 of 6 immunomodulatory (IM), 1 of 4 mesenchymal (M), 1 of 5 luminal androgen receptor (LAR) and 2 of 8 mesenchymal stem cell (MSL) showed lower frequencies of SOX10 expression. There was negative correlation between SOX10 and AR+ subtypes (P < .002). SOX10 was positively correlated with WT1 (P = .05). SOX10 did not show significant correlation with mammaglobin, GCDFP15, EGFR, CK5/6 and GATA3. SOX10 expression in the basal-like and unstable molecular subtypes supports the concept that these neoplasms show myoepithelial differentiation.
转录因子 SOX10 介导神经嵴衍生细胞的分化，免疫组织化学 (IHC) 主要用于黑色素瘤的诊断。SOX10 表达已在良性乳腺肌上皮细胞中得到证实。然而，关于其在三阴性乳腺癌 (TNBC) 中的表达的文献有限。本研究旨在研究 TNBC 中 SOX10+肿瘤的临床、病理和分子特征。对 48 例 TNBC 的组织微阵列进行 SOX10 表达检测。SOX10 表达与年龄、分级和分期等临床和病理特征相关。使用 Affymetrix 2.0 HTA 从福尔马林固定石蜡包埋 (FFPE) 标本中提取 RNA 进行基因表达分析。还评估了 SOX10 与雄激素受体 (AR)、WT1、大体囊性疾病液体蛋白 15 (GCDFP-15)、乳球蛋白、表皮生长因子受体 (EGFR)、CK5/6 和 GATA 转录因子 3 (GATA3) 的共表达。平均年龄为 59.38 岁（范围，28-90 岁）。总体而言，48 例中的 37.5%（18/48）为 SOX10+。SOX10 表达与患者年龄、分级或分期无关；10 例基底样 1 (BL1) 中有 6 例（60%）和 8 例不稳定 (UNS) 分子亚型中有 5 例为 SOX10+。BL2 中有 1 例、IM 中有 6 例、M 中有 4 例、LAR 中有 1 例和 MSL 中有 8 例中的 2 例显示 SOX10 表达频率较低。SOX10 与 AR+亚型呈负相关（P<0.002）。SOX10 与 WT1 呈正相关（P=0.05）。SOX10 与乳球蛋白、GCDFP15、EGFR、CK5/6 和 GATA3 无显著相关性。在基底样和不稳定分子亚型中 SOX10 的表达支持这些肿瘤表现出肌上皮分化的概念。
