Ateba Joël E T, Toghueo Rufin M K, Awantu Angelbert F, Mba'ning Brice M, Gohlke Sebastian, Sahal Dinkar, Rodrigues-Filho Edson, Tsamo Etienne, Boyom Fabrice F, Sewald Norbert, Lenta Bruno N
Department of Organic Chemistry, Faculty of Science, University of Yaoundé 1, P.O. Box 812, 237 Yaoundé, Cameroon.
Department of Biochemistry, Faculty of Science, University of Yaoundé I, Cameroon; P.O. Box 812, 237 Yaoundé, Cameroon.
J Fungi (Basel). 2018 Jun 12;4(2):70. doi: 10.3390/jof4020070.
There is continuing need for new and improved drugs to tackle malaria, which remains a major public health problem, especially in tropical and subtropical regions of the world. Natural products represent credible sources of new antiplasmodial agents for antimalarial drug development. Endophytes that widely colonize healthy tissues of plants have been shown to synthesize a great variety of secondary metabolites that might possess antiplasmodial benefits. The present study was carried out to evaluate the antiplasmodial potential of extracts from endophytic fungi isolated from against a chloroquine-resistant strain of ). Sixty-one fungal isolates with infection frequency of 67.77% were obtained from the bark of . Twelve selected isolates were classified into six different genera including , , , , and . Extracts from the 12 isolates were tested against , and nine showed good activity (IC < 10 μg·mL) with three fungi including (IC = 0.44 μg·mL), (IC = 0.2 μg·mL), and sp. (IC = 0.55 μg·mL) showing the highest promise. These three isolates were found to be less cytotoxic against the HEK293T cell line with selectivity indices ranging from 24.52 to 70.56. Results from this study indicate that endophytic fungi from are promising sources of hit compounds that might be further investigated as novel drugs against malaria. The chemical investigation of active extracts is ongoing.
仍然持续需要新的和改良的药物来应对疟疾，疟疾仍是一个重大的公共卫生问题，特别是在世界热带和亚热带地区。天然产物是抗疟疾药物开发中新的抗疟原虫药物的可靠来源。广泛定殖于植物健康组织中的内生菌已被证明能合成多种可能具有抗疟原虫益处的次生代谢产物。本研究旨在评估从[植物名称]分离的内生真菌提取物对氯喹抗性[疟原虫名称]菌株的抗疟原虫潜力。从[植物名称]树皮中获得了61株感染频率为67.77%的真菌分离株。12株选定的分离株被分为6个不同的属，包括[属名1]、[属名2]、[属名3]、[属名4]、[属名5]和[属名6]。对这12株分离株的提取物进行了抗[疟原虫名称]测试，9株显示出良好的活性（IC<10μg·mL），其中3株真菌包括[真菌名称1]（IC = 0.44μg·mL）、[真菌名称2]（IC = 0.2μg·mL）和[真菌名称3] sp.（IC = 0.55μg·mL）显示出最高的潜力。发现这3株分离株对HEK293T细胞系的细胞毒性较小，选择性指数范围为24.52至70.56。本研究结果表明，来自[植物名称]的内生真菌是有前景的先导化合物来源，可能作为抗疟疾新药进一步研究。活性提取物的化学研究正在进行中。
