局部淫羊藿素应用增强了牙周炎小型猪模型的牙周组织再生，缓解了局部炎症。
Local icariin application enhanced periodontal tissue regeneration and relieved local inflammation in a minipig model of periodontitis.
机构信息
Department of Periodontology, Capital Medical University School of Stomatology, Beijing, China.
Laboratory of Molecular Signaling and Stem Cells Therapy, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China.
出版信息
Int J Oral Sci. 2018 Jun 12;10(2):19. doi: 10.1038/s41368-018-0020-3.
Periodontitis is an inflammatory autoimmune disease. Treatment should alleviate inflammation, regulate the immune reaction and promote periodontal tissue regeneration. Icariin is the main active ingredient of Epimedii Folium, and it is a promising compound for the enhancement of mesenchymal stem cell function, promotion of bone formation, inhibition of bone resorption, alleviation of inflammation and regulation of immunity. The study investigated the effect of icariin on periodontal tissue regeneration in a minipig model of periodontitis. The minipig model of periodontitis was established. Icariin was injected locally. The periodontal clinical assessment index, a computed tomography (CT) scan, histopathology and enzyme-linked immune sorbent assay (ELISA) were used to evaluate the effects of icariin. Quantitative analysis results 12 weeks post-injection demonstrated that probing depth, gingival recession, attachment loss and alveolar bone regeneration values were (3.72 ± 1.18) mm vs. (6.56 ± 1.47) mm, (1.67 ± 0.59) mm vs. (2.38 ± 0.61) mm, (5.56 ± 1.29) mm vs. (8.61 ± 1.72) mm, and (25.65 ± 5.13) mm vs. (9.48 ± 1.78) mm in the icariin group and 0.9% NaCl group, respectively. The clinical assessment, CT scan, and histopathology results demonstrated significant enhancement of periodontal tissue regeneration in the icariin group compared to the 0.9% NaCl group. The ELISA results suggested that the concentration of interleukin-1 beta (IL-1β) in the icariin group was downregulated compared to the 0.9% NaCl group, which indicates that local injection of icariin relieved local inflammation in a minipig model of periodontitis. Local injection of icariin promoted periodontal tissue regeneration and exerted anti-inflammatory and immunomodulatory function. These results support the application of icariin for the clinical treatment of periodontitis.
牙周炎是一种炎症性自身免疫性疾病。治疗应减轻炎症、调节免疫反应并促进牙周组织再生。淫羊藿苷是淫羊藿的主要活性成分，是增强间充质干细胞功能、促进骨形成、抑制骨吸收、减轻炎症和调节免疫的有前途的化合物。本研究探讨了淫羊藿苷在牙周炎小型猪模型中对牙周组织再生的影响。建立了小型猪牙周炎模型，局部注射淫羊藿苷，通过牙周临床评估指标、计算机断层扫描（CT）扫描、组织病理学和酶联免疫吸附试验（ELISA）评估淫羊藿苷的作用。注射后 12 周的定量分析结果表明，探诊深度、牙龈退缩、附着丧失和牙槽骨再生值分别为（3.72±1.18）mm 比（6.56±1.47）mm、（1.67±0.59）mm 比（2.38±0.61）mm、（5.56±1.29）mm 比（8.61±1.72）mm 和（25.65±5.13）mm 比（9.48±1.78）mm，在淫羊藿苷组和 0.9%NaCl 组中，临床评估、CT 扫描和组织病理学结果表明，与 0.9%NaCl 组相比，牙周组织再生得到了显著增强。ELISA 结果表明，与 0.9%NaCl 组相比，淫羊藿苷组白细胞介素-1β（IL-1β）的浓度下调，这表明局部注射淫羊藿苷可减轻小型猪牙周炎模型中的局部炎症。局部注射淫羊藿苷促进牙周组织再生，并发挥抗炎和免疫调节作用。这些结果支持淫羊藿苷在牙周炎的临床治疗中的应用。
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