Madeira Jean-Paul, Alpha-Bazin Béatrice, Armengaud Jean, Duport Catherine
SQPOV, UMR0408, Avignon Université, INRA, F-84914 Avignon, France.
CEA, DRF/Joliot/DMTS/SPI, Li2D, Laboratory "Innovative technologies for Detection and Diagnostics", Bagnols-sur-Cèze F-30200, France.
Data Brief. 2018 Mar 10;18:394-398. doi: 10.1016/j.dib.2018.03.030. eCollection 2018 Jun.
Aerobic respiratory growth generates endogenous reactive oxygen species (ROS). ROS oxidize protein-bound methionine residues into methionine sulfoxide. Methionine sulfoxide reductases catalyze the reduction of methionine sulfoxide to methionine in proteins. Here, we use high-throughput nanoLC-MS/MS methodology to establish detailed maps of oxidized proteins from ATCC 14579 ΔpBClin15 and its mutant for which the methionine sulfoxide reductase AB gene () has been inactivated (Madeira et al., 2017) [1]. Lists of oxidized peptides and proteins identified at early exponential, late exponential and stationary growth phases are supplied in this article as data files. Raw data are deposited at the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifiers, PXD006169 and PDX006205 (http://www.ebi.ac/uk). Given the importance of methionine oxidation in several key cellular processes and its impact in the field of medical and food microbiology, this paper should be useful for further insightful redox studies in and its numerous relatives.
需氧呼吸生长会产生内源性活性氧（ROS）。ROS将蛋白质结合的甲硫氨酸残基氧化为甲硫氨酸亚砜。甲硫氨酸亚砜还原酶催化蛋白质中甲硫氨酸亚砜还原为甲硫氨酸。在此，我们使用高通量纳升液相色谱-串联质谱（nanoLC-MS/MS）方法，建立了来自ATCC 14579 ΔpBClin15及其甲硫氨酸亚砜还原酶AB基因（）已失活的突变体的氧化蛋白质详细图谱（马德拉等人，2017年）[1]。本文以数据文件形式提供了在指数生长早期、指数生长后期和稳定期鉴定出的氧化肽和蛋白质列表。原始数据通过PRIDE合作伙伴存储库存入蛋白质组交换联盟，数据集标识符为PXD006169和PDX006205（http://www.ebi.ac/uk）。鉴于甲硫氨酸氧化在几个关键细胞过程中的重要性及其在医学和食品微生物学领域的影响，本文应有助于对及其众多亲缘物种进行更深入的氧化还原研究。
