Department of Chemistry, RCSI, 123 St. Stephen's Green, Dublin 2, Ireland.
Chem Commun (Camb). 2018 Jun 26;54(52):7219-7222. doi: 10.1039/c8cc02135j.
The recognition of the biological, diagnostic and medical importance of exosomes has given rise to an urgent need for efficient labelling of these extracellular vesicles in ways that do not alter their inherent characteristics. We report for the first time an endogenous method to NIR-fluorescent labelled exosomes using an amphiphilic probe without the need for immunolabelling or synthetic or chromatographic manipulation of exosomes. Comparative analyses of labelled and unlabelled exosomes with NTA, AFM, flow cytometry and immunoblot analysis all show a high degree of similarity. Spectroscopic analysis and fluorescence imaging confirmed the ability to visualise purified NIR-exosomes.
外泌体的生物学、诊断学和医学重要性的认识，使得迫切需要以不改变其固有特性的方式对这些细胞外囊泡进行有效的标记。我们首次报道了一种使用两亲探针对近红外荧光标记外泌体的内源性方法，而无需对外泌体进行免疫标记或合成或色谱处理。用 NTA、原子力显微镜、流式细胞术和免疫印迹分析对标记和未标记的外泌体进行的比较分析均表明它们具有高度的相似性。光谱分析和荧光成像证实了对纯化的近红外外泌体进行可视化的能力。
