Edson C M, Hosler B A, Poodry C A, Schooley R T, Waters D J, Thorley-Lawson D A
Virology. 1985 Aug;145(1):62-71. doi: 10.1016/0042-6822(85)90201-6.
Varicella-zoster virus (VZV)-infected human foreskin fibroblasts synthesize viral glycoproteins of 125,000 (gp125), 118,000 (gp118), 92,000 (gp92), 63,000 (gp63), 59,000 (gp59), and 47,000 (gp47) Da. In biochemical studies, all of these VZV glycoproteins were shown to contain asparagine-linked (N-linked) oligosaccharide chains and, except for gp125 and gp47, to be sialoglycoproteins. Experiments with endo-beta-N-acetylglucosaminidase H (endo H) demonstrated that gp92 contained only complex type (endo H-resistant) N-linked glycosyl chains, while the other mature glycoproteins contained both high-mannose (endo H-sensitive) and complex-type oligosaccharides. Monoclonal antibodies recognizing multiple glycoproteins, gp63/gp125 or gp92/gp59/gp47, neutralized virus infection, suggesting the glycoproteins were important components of the virus envelope. This was confirmed for gp92/gp59/gp47 by immunoelectron microscopy, which revealed dense staining localized exclusively to the virion envelope and to the plasma membrane of virus-producer cells. The mature forms of all of these glycoproteins were also present in viral material isolated from vesicles of varicella and zoster patients, indicating that in infected individuals the viral glycoproteins are synthesized and processed in a manner similar to that in tissue culture cells.
水痘带状疱疹病毒（VZV）感染的人包皮成纤维细胞可合成分子量为125,000（gp125）、118,000（gp118）、92,000（gp92）、63,000（gp63）、59,000（gp59）和47,000（gp47）道尔顿的病毒糖蛋白。在生化研究中，所有这些VZV糖蛋白均显示含有天冬酰胺连接（N连接）的寡糖链，并且除gp125和gp47外，均为唾液酸糖蛋白。用内切β-N-乙酰氨基葡萄糖苷酶H（内切H）进行的实验表明，gp92仅含有复合型（内切H抗性）N连接糖基链，而其他成熟糖蛋白则同时含有高甘露糖型（内切H敏感型）和复合型寡糖。识别多种糖蛋白（gp63/gp125或gp92/gp59/gp47）的单克隆抗体可中和病毒感染，这表明这些糖蛋白是病毒包膜的重要成分。通过免疫电子显微镜对gp92/gp59/gp47进行了确认，结果显示密集染色仅定位于病毒体包膜和病毒产生细胞的质膜。所有这些糖蛋白的成熟形式也存在于从水痘和带状疱疹患者水疱中分离出的病毒物质中，这表明在受感染个体中，病毒糖蛋白的合成和加工方式与组织培养细胞中的相似。
