Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, I‑56126 Pisa, Italy.
Department of Clinical and Experimental Medicine, School of Medicine, University of Pisa, I‑56126 Pisa, Italy.
Mol Med Rep. 2018 Aug;18(2):1798-1803. doi: 10.3892/mmr.2018.9121. Epub 2018 Jun 1.
An increase in skin rashes or atopic dermatitis has been observed in individuals working with vanadium. However, to the best of our knowledge no in vivo or in vitro studies have evaluated the effect of exposure to vanadium in dermal fibroblasts. Cells viability and proliferation were assessed by WST‑1 assay, cells were treated with increasing concentrations of V2O5 (1, 10 and 100 nM). CXCL8 and CXCL11 concentrations were measured in the supernatants using an ELISA assay. V2O5 was not observed as having a significant effect on dermal fibroblast's viability and proliferation. However, it was revealed that V2O5 was able to induce the secretion of CXCL8 and CXCL11 chemokines into dermal fibroblasts. V2O5 synergistically increased the effect of interferon (IFN)γ on CXCL11 secretion. In addition, V2O5 synergistically increased the effect of the tumor necrosis factor α on CXCL8 secretion and abolished the inhibitory effect of IFNγ. V2O5 induction of CXCL8 and CXCL11 chemokines may lead to the appearance and perpetuation of an inflammatory reaction into the dermal tissue. Further studies are required to evaluate dermal integrity and manifestations in subjects occupationally exposed, or living in polluted areas.
据观察，接触钒的个体皮肤疹或特应性皮炎的发病率有所增加。但是，据我们所知，尚无研究评估过暴露于五氧化二钒对真皮成纤维细胞的影响。通过 WST-1 检测法评估细胞活力和增殖，用不同浓度的 V2O5（1、10 和 100 nM）处理细胞。采用 ELISA 检测法测量上清液中 CXCL8 和 CXCL11 的浓度。结果表明，V2O5 对真皮成纤维细胞的活力和增殖没有显著影响。然而，V2O5 能够诱导真皮成纤维细胞分泌趋化因子 CXCL8 和 CXCL11。V2O5 还协同增强了干扰素 (IFN)γ对 CXCL11 分泌的作用。此外，V2O5 协同增强了肿瘤坏死因子 α对 CXCL8 分泌的作用，并消除了 IFNγ 的抑制作用。V2O5 诱导的 CXCL8 和 CXCL11 趋化因子的产生可能导致真皮组织中炎症反应的出现和持续存在。需要进一步研究评估职业暴露或生活在污染地区的人群的皮肤完整性和表现。
