活体巨噬细胞内金黄色葡萄球菌可能是抗生素治疗失败的原因：在体内人工血管材料感染的小鼠模型中的观察结果。
Live intramacrophagic Staphylococcus aureus as a potential cause of antibiotic therapy failure: observations in an in vivo mouse model of prosthetic vascular material infections.
机构信息
Institut des Sciences Moléculaires d'Orsay (ISMO), CNRS, Université Paris-Sud, Université Paris-Saclay, F-91405 Orsay, France.
EA 3826, Université Nantes, Faculté Médecine, Nantes, France.
出版信息
J Antimicrob Chemother. 2018 Sep 1;73(9):2418-2421. doi: 10.1093/jac/dky205.
OBJECTIVES
To evaluate the significant role played by biofilms during prosthetic vascular material infections (PVMIs).
METHODS
We developed an in vivo mouse model of Staphylococcus aureus PVMI allowing its direct observation by confocal microscopy to describe: (i) the structure of biofilms developed on Dacron® vascular material; (ii) the localization and effect of antibiotics on these biostructures; and (iii) the interaction between bacteria and host tissues and cells during PVMI.
RESULTS
In this model we demonstrated that the biofilm structures are correlated to the activity of antibiotics. Furthermore, live S. aureus bacteria were visualized inside the macrophages present at the biofilm sites, which is significant as antibiotics do not penetrate these immune cells.
CONCLUSIONS
This intracellular situation may explain the limited effect of antibiotics and also why PVMIs can relapse after antibiotic therapy.
目的
评估生物膜在人工血管材料感染（PVMIs）中所起的重要作用。
方法
我们开发了一种金黄色葡萄球菌 PVMIs 的体内小鼠模型，通过共聚焦显微镜直接观察来描述：（i）在 Dacron®血管材料上形成的生物膜的结构；（ii）抗生素在这些生物结构上的定位和作用；以及（iii）在 PVMIs 期间细菌与宿主组织和细胞之间的相互作用。
结果
在该模型中，我们证明了生物膜结构与抗生素的活性相关。此外，活的金黄色葡萄球菌细菌在生物膜部位存在的巨噬细胞内被可视化，这一点很重要，因为抗生素不能穿透这些免疫细胞。
结论
这种细胞内情况可能解释了抗生素效果有限的原因，也是 PVMIs 在抗生素治疗后可能复发的原因。
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