Gal A, Nahon J L, Lucotte G, Erdos T, Sala-Trepat J M
Biochem Genet. 1985 Apr;23(3-4):257-66. doi: 10.1007/BF00504323.
Two types of variant EcoRI restriction enzyme patterns of albumin-gene DNA fragments are found in different inbred strains of rats and reflect allelic polymorphism. The structural basis of the two allelic forms has been analyzed by mapping the EcoRI fragments using cloned albumin cDNA probes corresponding to the 5' or 3' end of the rat albumin mRNA and different genomic subclones. Additional restriction fragment length polymorphism has been detected using the restriction endonucleases HindIII and MspI. The results suggest that the two allelic variants differ from each other by multiple cleavage-site variations (base-pair substitutions) and by an insertion or deletion of DNA sequences. An extensive DNA sequence variation appears to exist between the two forms of the albumin gene; we have estimated that as much as 4% of the nucleotides in this region varied between the two alleles. All of this genetic variation is found in the intervening sequences of the gene and has no phenotypic manifestation.
在不同的近交系大鼠中发现了两种白蛋白基因DNA片段的变异型EcoRI限制性酶切图谱，这反映了等位基因多态性。通过使用与大鼠白蛋白mRNA 5'或3'末端相对应的克隆白蛋白cDNA探针以及不同的基因组亚克隆对EcoRI片段进行定位，分析了两种等位基因形式的结构基础。使用限制性内切酶HindIII和MspI检测到了额外的限制性片段长度多态性。结果表明，这两种等位基因变体在多个切割位点变异（碱基对替换）以及DNA序列的插入或缺失方面彼此不同。两种形式的白蛋白基因之间似乎存在广泛的DNA序列变异；我们估计这两个等位基因之间该区域多达4%的核苷酸存在差异。所有这些遗传变异都存在于基因的间隔序列中，且没有表型表现。
