Huybrechts-Godin G, Peeters-Joris C, Vaes G
Biochim Biophys Acta. 1985 Jul 30;846(1):51-4. doi: 10.1016/0167-4889(85)90109-0.
Rabbit bone marrow-derived macrophages in culture produce and release a soluble factor that activates collagenase secretion and collagen degradation by cultured skin fibroblasts from either rabbit, mouse or human origin. The factor is heat-labile and is inactivated by phenylglyoxal. After gel filtration, it is recovered in both an apparent high-Mr (67000-76000) and a low-Mr (9000-14000) form. Chromatography on cation exchangers suggests two molecular species with different charge properties. These characteristics are compatible with known properties of rabbit interleukin 1.
培养的兔骨髓源性巨噬细胞产生并释放一种可溶性因子，该因子可激活来自兔、小鼠或人类的培养皮肤成纤维细胞的胶原酶分泌和胶原降解。该因子对热不稳定，可被苯乙二醛灭活。凝胶过滤后，它以表观高Mr（67000 - 76000）和低Mr（9000 - 14000）两种形式回收。在阳离子交换剂上进行色谱分析表明存在两种具有不同电荷性质的分子种类。这些特征与兔白细胞介素1的已知性质相符。
