Heath J K, Meikle M C, Atkinson S J, Reynolds J J
Biochim Biophys Acta. 1984 Aug 21;800(3):301-5. doi: 10.1016/0304-4165(84)90409-4.
Unstimulated monolayer cultures of confluent rabbit periosteal fibroblasts synthesize a factor that stimulates bone resorption in vitro. Furthermore it stimulates rabbit chondrocytes and mouse osteoblasts to synthesize collagenase. The factor has no effect on dead bone in culture, and its activity on live bone is mediated principally by osteoclasts, since it is 75% inhibited by salmon calcitonin. Characterization of the factor by gel filtration and isoelectric focusing indicates an Mr in the range 15000-25000 and a pI corresponding to approx. pH 4.7. These biological and physiochemical properties are similar to those reported for a factor released by peripheral blood monocytes. However, whereas human monocyte factor in both the crude and partially-purified state exhibits interleukin-1 activity, crude and fractionated periosteal fibroblast-conditioned medium does not. This is the first report of a conditioned medium containing a molecule like the monocyte-factor which appears to have no interleukin 1 activity. The factor may be synthesized by a wide range of cell types, and could have an important role in mediating connective tissue degradation during both physiological and pathological resorption.
汇合的兔骨膜成纤维细胞的未刺激单层培养物合成一种在体外刺激骨吸收的因子。此外，它还刺激兔软骨细胞和小鼠成骨细胞合成胶原酶。该因子对培养中的死骨没有作用，其对活骨的活性主要由破骨细胞介导，因为它被鲑鱼降钙素抑制了75%。通过凝胶过滤和等电聚焦对该因子进行表征表明，其分子量在15000 - 25000范围内，等电点约为pH 4.7。这些生物学和物理化学性质与外周血单核细胞释放的一种因子所报道的性质相似。然而，粗制和部分纯化状态的人单核细胞因子都表现出白细胞介素-1活性，而粗制和分级的骨膜成纤维细胞条件培养基则没有。这是关于一种条件培养基的首次报道，该培养基含有一种类似单核细胞因子的分子，但似乎没有白细胞介素-1活性。该因子可能由多种细胞类型合成，并且在生理和病理吸收过程中介导结缔组织降解方面可能具有重要作用。
