Department of Biology, 1878 Campus Delivery, Colorado State University, Fort Collins, CO 80523-1878, USA.
Department of Biology, 1878 Campus Delivery, Colorado State University, Fort Collins, CO 80523-1878, USA.
Plant Sci. 2018 Aug;273:13-22. doi: 10.1016/j.plantsci.2018.04.005. Epub 2018 Apr 11.
Plant synthetic biology is a rapidly emerging field that aims to engineer genetic circuits to function in plants with the same reliability and precision as electronic circuits. These circuits can be used to program predictable plant behavior, producing novel traits to improve crop plant productivity, enable biosensors, and serve as platforms to synthesize chemicals and complex biomolecules. Herein we introduce the importance of developing orthogonal plant parts and the need for quantitative part characterization for mathematical modeling of complex circuits. In particular, transfer functions are important when designing electronic-like genetic controls such as toggle switches, positive/negative feedback loops, and Boolean logic gates. We then discuss potential constraints and challenges in synthetic regulatory circuit design and integration when using plants. Finally, we highlight current and potential plant synthetic regulatory circuit applications.
植物合成生物学是一个迅速崛起的领域，旨在通过工程遗传回路，使它们在植物中像电子电路一样可靠和精确地运行。这些回路可用于编程可预测的植物行为，产生新的特性以提高作物的生产力，实现生物传感器，并作为合成化学物质和复杂生物分子的平台。在此，我们介绍了开发正交植物元件的重要性，以及为复杂回路的数学建模进行定量元件表征的必要性。特别是，当设计类似于电子的遗传控制，如 toggle 开关、正/负反馈回路和布尔逻辑门时，传递函数很重要。然后，我们讨论了在使用植物时合成调控回路设计和集成中的潜在限制和挑战。最后，我们强调了当前和潜在的植物合成调控回路的应用。
