Goindin D, Cannet A, Delannay C, Ramdini C, Gustave J, Atyame C, Vega-Rúa A
Laboratory of Medical Entomology, Environment and Health Unit, Institut Pasteur de la Guadeloupe, 97183, Les Abymes, Guadeloupe, France.
National Center of Expertise on Vectors (CNEV), Centre IRD France Sud 911 avenue Agropolis BP 64501, 34394, Montpellier cedex 5, France.
Acta Trop. 2018 Sep;185:314-317. doi: 10.1016/j.actatropica.2018.06.011. Epub 2018 Jun 13.
Guadeloupe islands are threatened by several mosquito-borne viruses such as Dengue, Chikungunya, Zika and West Nile virus. It appears essential to look for alternative mosquito control methods such as the incompatible insect technique (ITT) aiming at sterilizing wild females by inundative releases of incompatible males. Before considering the implementation of such a strategy, the characterization of genetic diversity of the endocellular bacterium Wolbachia regarding the local mosquito populations is a critical issue. Here, for the first time, we describe the prevalence and diversity of Wolbachia in natural populations of three mosquito species from Guadeloupe: Aedes aegypti, Aedes taeniorhynchus and Culex quinquefasciatus. The detection of Wolbachia in natural Ae. aegypti, Ae. taeniorhynchus and Cx. quinquefasciatus populations was conducted by studying Wolbachia 16S ribosomal RNA gene using a TaqMan quantitative real-time PCR and results were confirmed by conventional PCR and sequencing. In addition, molecular typing of wPip strains in Cx. quinquefasciatus was done by PCR-RFLP. We did not find Wolbachia infection in any of Ae. aegypti and Ae. taeniorhynchus studied populations. Natural Wolbachia infection was detected in Cx. quinquefasciatus with prevalence varying from 79.2% to 95.8%. In addition, no polymorphism was found between the Wolbachia strains infecting Cx. quinquefasciatus specimens, all carrying an infection from the same Wolbachia genetic wPip-I group. These results pave the way for the evaluation of the feasibility of IIT programs to fight against these medically-important mosquito species in Guadeloupe.
瓜德罗普群岛受到几种蚊媒病毒的威胁，如登革热、基孔肯雅热、寨卡病毒和西尼罗河病毒。寻找替代的蚊虫控制方法，如不相容昆虫技术（ITT），通过大量释放不相容雄蚊来使野生雌蚊绝育，似乎至关重要。在考虑实施这种策略之前，针对当地蚊虫种群对内细胞细菌沃尔巴克氏体的遗传多样性进行表征是一个关键问题。在此，我们首次描述了瓜德罗普三种蚊虫自然种群中沃尔巴克氏体的流行情况和多样性：埃及伊蚊、带喙伊蚊和致倦库蚊。通过使用TaqMan定量实时PCR研究沃尔巴克氏体16S核糖体RNA基因，对埃及伊蚊、带喙伊蚊和致倦库蚊自然种群中的沃尔巴克氏体进行检测，结果通过常规PCR和测序得到证实。此外，通过PCR-RFLP对致倦库蚊中的wPip菌株进行分子分型。在我们研究的任何埃及伊蚊和带喙伊蚊种群中均未发现沃尔巴克氏体感染。在致倦库蚊中检测到自然沃尔巴克氏体感染，感染率在79.2%至95.8%之间。此外，感染致倦库蚊标本中的沃尔巴克氏体菌株之间未发现多态性，所有菌株均携带来自同一沃尔巴克氏体遗传wPip-I组的感染。这些结果为评估不相容昆虫技术项目在瓜德罗普对抗这些具有医学重要性的蚊虫物种的可行性铺平了道路。
