Kanthaliya Bhanupriya, Joshi Abhishek, Arora Jaya, Alqahtani Mashael Daghash, Abd Allah Elsayed Fathi
Laboratory of Biomolecular Technology, Department of Botany, Mohanlal Sukhadia University, Udaipur 313001, Rajasthan, India.
Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia.
Plants (Basel). 2023 Mar 14;12(6):1300. doi: 10.3390/plants12061300.
contains a wide range of bioactive compounds, including polyphenols, alkaloids, and phytosterols, which make it valuable to the pharmaceutical and food industries. Elicitor compounds trigger the defense mechanisms in plants and are widely used to increase the yield of bioactive molecules in in vitro cultures. The present study was conducted to evaluate the effects of different concentrations of biotic elicitors such as yeast extract (YE), pectin (PEC), and alginate (ALG) on growth, antioxidant activity, and metabolite accumulation in in vitro propagated shoots of . The elicitors applied to shoot cultures of significantly increased biomass (shoot number, fresh weight, and dry weight), and metabolites such as protein, carbohydrates, chlorophyll, total phenol (TP), and total flavonoid (TF) contents, as well as antioxidant activity compared to untreated control. Biomass, TP, and TF contents, as well as antioxidant activity, were most significant in cultures treated with 100 mg/L PEC. In contrast, chlorophyll, protein, and carbohydrate increased most in cultures treated with 200 mg/L ALG. Application of 100 mg/L of PEC led to the accumulation of high amounts of isoflavonoids including puerarin (220.69 μg/g), daidzin (2935.55 μg/g), genistin (5612 μg/g), daidzein (479.81 μg/g), and biochanin-A (111.511 μg/g) as analyzed by high-performance liquid chromatography (HPLC). Total isoflavonoids content of 100 mg/L PEC treated shoots was obtained as 9359.56 μg/g, 1.68-fold higher than in vitro propagated shoots without elicitors (5573.13 μg/g) and 2.77-fold higher than shoots of the mother plant (3380.17 μg/g). The elicitor concentrations were optimized as 200 mg/L YE, 100 mg/L PEC, and 200 mg/L ALG. Overall, this study showed that the application of different biotic elicitors resulted in better growth, antioxidant activity, and accumulation of metabolites in , which could lead to obtaining phytopharmaceutical advantages in the future.
含有多种生物活性化合物，包括多酚、生物碱和植物甾醇，这使其对制药和食品工业具有重要价值。激发子化合物可触发植物的防御机制，并广泛用于提高体外培养中生物活性分子的产量。本研究旨在评估不同浓度的生物激发子，如酵母提取物（YE）、果胶（PEC）和藻酸盐（ALG），对[植物名称]体外繁殖芽的生长、抗氧化活性和代谢物积累的影响。与未处理的对照相比，应用于[植物名称]芽培养物的激发子显著增加了生物量（芽数、鲜重和干重）以及蛋白质、碳水化合物、叶绿素、总酚（TP）和总黄酮（TF）含量等代谢物，以及抗氧化活性。在100 mg/L PEC处理的培养物中，生物量、TP和TF含量以及抗氧化活性最为显著。相比之下，叶绿素、蛋白质和碳水化合物在200 mg/L ALG处理的培养物中增加最多。通过高效液相色谱（HPLC）分析，应用100 mg/L的PEC导致大量异黄酮的积累，包括葛根素（220.69 μg/g）、大豆苷（2935.55 μg/g）、染料木苷（5612 μg/g）、大豆苷元（479.81 μg/g）和鹰嘴豆芽素A（111.511 μg/g）。100 mg/L PEC处理的芽中总异黄酮含量为9359.56 μg/g，比未添加激发子的体外繁殖芽（5573.13 μg/g）高1.68倍，比母株芽（3380.17 μg/g）高2.77倍。激发子浓度优化为200 mg/L YE、100 mg/L PEC和200 mg/L ALG。总体而言，本研究表明，应用不同的生物激发子可使[植物名称]的生长、抗氧化活性和代谢物积累更好，这可能在未来带来植物药优势。
