Department of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center, Medical University, Graz, Austria.
Research Unit for Tissue Regeneration, Repair, and Reconstruction, Division of Plastic, Aesthetic and Reconstructive Surgery, Department of Surgery, Medical University, Graz, Austria.
Placenta. 2018 May;65:37-46. doi: 10.1016/j.placenta.2018.04.004. Epub 2018 Apr 10.
Mesenchymal stem/stromal cells derived from human term placentas (PMSCs) are novel therapeutic agents and more topical than ever. Here we evaluated the effects of three types of PMSCs on wound healing in an in vivo mouse model: Amnion-derived MSCs (AMSCs), blood vessel-derived MSCs (BV-MSCs) from the chorionic plate and Wharton's jelly-derived MSCs (WJ-MSCs) from the umbilical cord.
We topically applied PMSCs onto skin wounds in mice using the dermal substitute Matriderm as carrier and evaluated wound healing parameters. In addition, we investigated the effects of all PMSC types under co-application with placental endothelial cells (PLECs). After 8 days, we compared the percent of wound closure and the angiogenic potential between all groups.
AMSCs, BV-MSCs and WJ-MSCs significantly induced a faster healing and a higher number of blood vessels in the wound when compared to controls (Matriderm-alone). PLECs did not further improve the advantageous effects of PMSC-treatment. Quantitative data and 3D analysis by high resolution episcopic microscopy confirmed a lower density of vessels in Matriderm/PMSCs/PLECs co-application compared to Matriderm/PMSCs treatment.
Results indicate that all three PMSC types exert similar beneficial effects on wound closure and neovascularization in our mouse model.
Using Matriderm as carrier for PMSCs propagates rapid cell migration towards the wound area that allows a fast and clinically practicable method for stem cell application.
These promising effects warrant further investigation in clinical trials.
来源于人足月胎盘的间充质干细胞（PMSC）是新型治疗剂，比以往任何时候都更受关注。在此，我们评估了三种类型的 PMSC（羊膜来源的 MSC [AMSC]、源自绒毛板的血管来源的 MSC [BV-MSC]和源自脐带华通氏胶的 MSC [WJ-MSC]）在体内小鼠模型中对伤口愈合的影响。
我们使用真皮替代物 Matriderm 作为载体，将 PMSC 局部应用于小鼠皮肤伤口，并评估伤口愈合参数。此外，我们还研究了所有 PMSC 类型与胎盘内皮细胞（PLEC）共同应用的效果。8 天后，我们比较了所有组之间的伤口闭合百分比和血管生成潜力。
与对照组（仅 Matriderm）相比，AMSC、BV-MSC 和 WJ-MSC 明显诱导更快的愈合和更多的血管生成。PLEC 并没有进一步提高 PMSC 治疗的有利效果。高分辨率共聚焦显微镜的定量数据和 3D 分析证实，Matriderm/PMSC/PLEC 共处理的血管密度低于 Matriderm/PMSC 处理。
结果表明，三种 PMSC 类型在我们的小鼠模型中对伤口闭合和新生血管化均具有相似的有益作用。
使用 Matriderm 作为 PMSC 的载体可促进细胞快速迁移到伤口区域，从而为干细胞应用提供快速且在临床上可行的方法。
这些有希望的效果值得在临床试验中进一步研究。
